Alteration of the Cortical Actin Cytoskeleton Deregulates Ca2+ Signaling, Monospermic Fertilization, and Sperm Entry 英文参考文献.docVIP

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2017-05-11 发布于上海
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Alteration of the Cortical Actin Cytoskeleton Deregulates Ca2+ Signaling, Monospermic Fertilization, and Sperm Entry 英文参考文献.doc

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AlterationoftheCorticalActinCytoskeletonDeregulatesCa2Signaling,MonospermicFertilization,andSpermEntry英文参考文献

AlterationoftheCorticalActinCytoskeletonDeregulates Ca Signaling,MonospermicFertilization,andSperm Entry 2+ A.Puppo,JongT.Chun,GiovanniGragnaniello,EzioGarante,LuigiaSantella* StazioneZoologicaAntonDohrn,VillaComunale,Napoli,Italy Abstract Background:Whenpreparingforfertilization,oocytesundergomeioticmaturationduringwhichstructuralchangesoccur in the endoplasmic reticulum (ER) that lead to a more efficient calcium response. During meiotic maturation and subsequent fertilization, the actin cytoskeleton also undergoes dramatic restructuring. We have recently observed that rearrangementsoftheactincytoskeletoninducedbyactin-depolymerizingagents,orbyactin-bindingproteins,strongly 2+ modulate intracellular calcium (Ca ) signals during the maturation process. However, the significance of the dynamic changesinF-actinwithinthefertilizedegghasbeenlargelyunclear. Methodology/Principal Findings: We have measured changes in intracellular Ca2+ signals and F-actin structures during fertilization. We also report theunexpected observation that theconventional antagonist of the InsP3 receptor, heparin, hyperpolymerizesthecorticalactincytoskeletoninpostmeioticeggs.Usingheparinandotherpharmacologicalagentsthat eitherhypo-orhyperpolymerizethecorticalactin,wedemonstratethatnearlyallaspectsofthefertilizationprocessare profoundlyaffectedbythedynamicrestructuringoftheeggcorticalactincytoskeleton. Conclusions/Significance:Ourfindingsidentifyimportantrolesforsubplasmalemmalactinfibersintheprocessofsperm- egg interaction and in the subsequent events related to fertilization: the generation of Ca2+ signals, sperm penetration, corticalgranuleexocytosis,andtheblocktopolyspermy. Citation:PuppoA,ChunJT,GragnanielloG,GaranteE,SantellaL(2008)AlterationoftheCorticalActinCytoskeletonDeregulatesCa2+Signaling,Monospermic Fertilization,andSpermEntry.PLoSONE3(10):e3588.doi:10.1371/journal.pone.0003588 Editor:Karl-WilhelmKoch,UniversityofOldenburg,Germany ReceivedAugust19,2008;AcceptedOctober10,2008;Publis