An Endogenous Electron Spin Resonance (ESR) Signal Discriminates Nevi from Melanomas in Human Specimens A Step Forward in Its Diagnostic Application 英文参考文献.docVIP

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An Endogenous Electron Spin Resonance (ESR) Signal Discriminates Nevi from Melanomas in Human Specimens A Step Forward in Its Diagnostic Application 英文参考文献.doc

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An Endogenous Electron Spin Resonance (ESR) Signal Discriminates Nevi from Melanomas in Human Specimens A Step Forward in Its Diagnostic Application 英文参考文献

AnEndogenousElectronSpinResonance(ESR)Signal DiscriminatesNevifromMelanomasinHuman Specimens:AStepForwardinItsDiagnosticApplication EleonoraCesareo1,LiudmilaKorkina1*,GerardinoD’Errico2,3,GiuseppeVitiello2,3 ,Maria SimonaAguzzi4,FrancescaPassarelli5,JensZ.Pedersen6,AntonioFacchiano4* 1LaboratoryofTissueEngineeringSkinPathophysiology,IstitutoDermopaticodell’ImmacolataIDI-IRCCS,Rome,Italy,2DepartmentofChemicalSciences,Universityof Naples ‘‘Federico II’’, Naples, Italy, 3Consorzio per lo Sviluppo dei Sistemi a Grande Interfase (CSGI), Florence, Italy, 4Laboratory of Vascular Pathology, Istituto Dermopaticodell’Immacolata(IDI-IRCCS),Rome,Italy,5HistopathologyService,IstitutoDermopaticodell’Immacolata(IDI-IRCCS),Rome,Italy,6DepartmentofBiology, UniversityofTorVergata,Rome,Italy Abstract Given the specific melanin-associated paramagnetic features, the Electron Spin Resonance (ESR, called also Electron Paramagnetic Resonance, EPR) analysis has been proposed as a potential tool for non-invasive melanoma diagnosis. However,studiescomparinghumanmelanomatissuestothemostappropriatephysiologicalcounterpart(nevi)havenot beenperformed,andESRdirectcorrelationwithmelanomaclinicalfeatureshasneverbeeninvestigated.ESRspectrumwas obtainedfrommelanomaandnon-melanomacell-culturesaswellasmousemelanomaandnon-melanomatissuesandan endogenousESRsignal(g=2.005)wasfoundinhumanmelanomacellsandinprimarymelanomatissuesexplantedfrom mice, while it was always absent in non-melanoma samples. These characteristics of the measured ESR signal strongly suggested its connection with melanin. Quantitative analyses were then performed on paraffin-embedded human melanoma and nevus sections, and validated on an independent larger validation set, for a total of 112 sections (52 melanomas, 60 nevi). The ESR signal was significantly higher in melanomas (p=0.0002) and was significantly different between‘‘LowBreslow’sand‘‘HighBreslow’s’’depthmelanomas(p,0.0001).AdirectcorrelationbetweenESRsignaland Breslow’s dept