An Expanded Self-Antigen Peptidome Is Carried by the Human Lymph As Compared to the Plasma 英文参考文献.docVIP
- 1、本文档共10页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
An Expanded Self-Antigen Peptidome Is Carried by the Human Lymph As Compared to the Plasma 英文参考文献
AnExpandedSelf-AntigenPeptidomeIsCarriedbythe
HumanLymphAsComparedtothePlasma
CristinaC.Clement1,ElviraS.Cannizzo1,Maria-DorotheaNastke2,RanjitSahu1,WaldemarOlszewski3,
NormanE.Miller4,LawrenceJ.Stern2,LauraSantambrogio1,5*
1DepartmentofPathology,AlbertEinsteinCollegeofMedicine,NewYork,NewYork,UnitedStatesofAmerica,2DepartmentofPathology,UniversityofMassachusetts
Medical School, Worcester, Massachusetts, United States of America, 3Department of Surgical Research and Transplantology, Polish Academy of Sciences, Warsaw,
Poland,4MagdalenCollege,UniversityofOxford,Oxford,UnitedKingdom,5DepartmentofMicrobiologyandImmunology,AlbertEinsteinCollegeofMedicine,New
York,NewYork,UnitedStatesofAmerica
Abstract
Background: The pre-nodal afferent lymph is the fluid which directly derives from the extracellular milieu from every
parenchymal organ and, as it continues to circulate between the cells, it collects products deriving from the organ
metabolism/catabolism. A comprehensive qualitative and quantitative investigation of the self-antigenic repertoire
transportedbythehumanlymphisstillmissing.
Methodology/PrincipalFindings:AmajordifferencebetweenlymphandplasmacouldbevisualizedbyFPLCand2Dgelin
theamountoflowmolecularweightproductscorrespondingtopeptidefragments.Naturallyprocessedpeptidesinnormal
pre-nodal human lymph were then fractionated by HPLC and characterized by multidimensional mass spectrometry.
Analysis of more then 300 sequences identified self-peptides derived from both intracellular and extracellular proteins
revealing the variety of catabolic products transported by human lymph. Quantitative analysis established that at least
someofthesepeptidesarepresentinthecirculatinglymphinnanomolarconcentration.
Conclusions/Significance:Thepeptidome,generatedbyphysiologicaltissuecatabolismandtransportedbythepre-nodal
lymph,isinadditiontotheself-peptidomegeneratedinendosomalcompartment.Unlikeselfantigenprocessedbylocalor
nodalAPC,whichmostlyproduceepitopesconstrainedbytheendoso
您可能关注的文档
- An Economic Evaluation of Venous Thromboembolism Prophylaxis Strategies in Critically Ill Trauma Patients at Risk of Bleeding 英文参考文献.doc
- An Ecological Analysis of the Herbivory-Elicited JA Burst and Its Metabolism Plant Memory Processes and Predictions of the Moving Target Model 英文参考文献.doc
- An Effective Assessment of Simvastatin-Induced Toxicity with NMR-Based Metabonomics Approach 英文参考文献.doc
- An Ecological Study of the Determinants of Differences in 2009 Pandemic Influenza Mortality Rates between Countries in Europe 英文参考文献.doc
- An Ecosystem Evaluation Framework for Global Seamount Conservation and Management 英文参考文献.doc
- An Effective Strategy for a Whole-Cell Biosensor Based on Putative Effector Interaction Site of the Regulatory DmpR Protein 英文参考文献.doc
- An E2F1-Mediated DNA Damage Response Contributes to the Replication of Human Cytomegalovirus 英文参考文献.doc
- An Efficient Kinetic Model for Assemblies of Amyloid Fibrils and Its Application to Polyglutamine Aggregation 英文参考文献.doc
- An Efficient Paradigm for Genetic Epidemiology Cohort Creation 英文参考文献.doc
- An Efficient Strategy for Broad-Range Detection of Low Abundance Bacteria without DNA Decontamination of PCR Reagents 英文参考文献.doc
文档评论(0)