ANK, a Host Cytoplasmic Receptor for the Tobacco mosaic virus Cell-to-Cell Movement Protein, Facilitates Intercellular Transport through Plasmodesmata 英文参考文献.docVIP

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ANK, a Host Cytoplasmic Receptor for the Tobacco mosaic virus Cell-to-Cell Movement Protein, Facilitates Intercellular Transport through Plasmodesmata 英文参考文献.doc

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ANK, a Host Cytoplasmic Receptor for the Tobacco mosaic virus Cell-to-Cell Movement Protein, Facilitates Intercellular Transport through Plasmodesmata 英文参考文献

ANK,aHostCytoplasmicReceptorfortheTobacco mosaicvirusCell-to-CellMovementProtein,Facilitates IntercellularTransportthroughPlasmodesmata ShokoUeki*,RomanSpektor,DanielleM.Natale,VitalyCitovsky DepartmentofBiochemistryandCellBiology,StateUniversityofNewYork,StonyBrook,NewYork,UnitedStatesofAmerica Abstract Plasmodesma (PD) is a channel structure that spans the cell wall and provides symplastic connection between adjacent cells. Variousmacromolecules are knownto be transported through PD in ahighly regulated manner,and plant viruses utilizetheirmovementproteins(MPs)togatethePDtospreadcell-to-cell.ThemechanismbywhichMPmodifiesPDto enableintercelluartrafficremainsobscure,duetothelackofknowledgeaboutthehostfactorsthatmediatetheprocess. Here,wedescribethefunctionalinteractionbetweenTobaccomosaicvirus(TMV)MPandaplantfactor,anankyrinrepeat containingprotein(ANK),duringtheviralcell-to-cellmovement.Weutilizedareversegeneticsapproachtogaininsightinto thepossibleinvolvementofANKinviralmovement.Tothisend,ANKoverexpressorandsuppressorlinesweregenerated, andthemovementofMPwastested.MPmovementwasfacilitatedintheANK-overexpressingplants,andreducedinthe ANK-suppressingplants,demonstratingthatANKisahostfactorthatfacilitatesMPcell-to-cellmovement.Also,theTMV localinfectionwaslargelydelayedintheANK-suppressinglines,whileenhancedintheANK-overexpressinglines,showing that ANK is crucially involved in the infection process. Importantly, MP interacted with ANK at PD. Finally, simultaneous expressionofMPandANKmarkedlydecreasedthePDlevelsofcallose,b-1,3-glucan,whichisknowntoactasamolecular sphincterforPD.Thus,theMP-ANKinteractionresultsinthedownregulationofcalloseandincreasedcell-to-cellmovement of the viral protein. These findings suggest that ANK represents a host cellular receptor exploited by MP to aid viral movementbygatingPDthroughrelaxationoftheircallosesphincters. Citation: Ueki S, Spektor R, Natale DM, Citovsky V (2010) ANK, a Host Cytoplasmic Receptor for the Tobacco mosaic virus Ce