脂肪酶和青霉素酰化酶催化合成手性化合物的研究进展.pdfVIP

学兔兔 化 工 进 展 201 1年第30卷第7期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ·l577· e^ ， a， 0 《进展与述评； 气 脂肪酶和青霉素酰化酶催化合成手性化合物的研究进展 薛 屏，曹雪荣 (宁夏大学能源化重点实验室，宁夏 银川750021) 摘 要：生物酶催化合成手性药物和中间体具有高效、节能和环境友好等突出的优势，本文从提高催化反应效 率的角度，评述了非水相酶催化反应的溶剂效应——溶剂体系对酶的活性和对映选择性的调控作用；介绍了动力 学控制合成 内酰胺类抗生素的不同溶剂体系，包括有机物一水共溶剂体系、有机物一水不共溶剂体系、反胶束体 系和双水相体系。提出利用溶剂效应调控动力学合成体系，会有效提高酶催化合成 B 内酰胺类抗生素的产率和 合成与水解比S／H值，从而实现底物的有效利用。 关键词：溶剂效应；脂肪酶；青霉素G酰化酶；手性化合物；对映选择性 中图分类号：O 643．4 文献标志码：A 文章编号：1000—6613(2011)07—1577—07 Solvent effect on catalytic synthesis of chiral compouds with lipase and penicillin G acylase XUEPing，CAOXuerong (Key Laboratory ofEnergy Resources and ChemicaI Engineering，Ningxia University Yinchuan 750021，Ningxia，China) Abstract：Synthesis of chiral drugs and intermediates catalyzed by enzyme has high efficiency，energy saving and environment—friendliness advantages．This paper reviews the solvent effect on the enzymatic reaction in non—aqueous media with lipases，namely，the activity and enantioselectivitv Of enzyme adjusted by solvent system to improve the efficiency of catalytic reaction．The kinetically controlled synthesis of D—lactam antibiotics with penicillin G acylase was introduced in different solvent systems，including the organic—water miscible solvents，organic—water immiscible solvents， reverse micelles and aqueous two—phase system．The yield of kinetically controlled synthesis of p lactam antibiotics and synthesis／hydrolysis ratio(S／H)could be enhanced by designing an effective