Arginine Cofactors on the Polymerase Ribozyme 英文参考文献.docVIP

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Arginine Cofactors on the Polymerase Ribozyme 英文参考文献.doc

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Arginine Cofactors on the Polymerase Ribozyme 英文参考文献

ArginineCofactorsonthePolymeraseRibozyme ChengguoYao,JaninaE.Moretti,PeterE.Struss,JunaidA.Spall,UlrichF.Mu¨ller* DepartmentofChemistryandBiochemistry,UniversityofCalifornia,SanDiego,LaJolla,California,UnitedStatesofAmerica Abstract TheRNAworldhypothesisstatesthattheearlyevolutionoflifewentthroughastageinwhichRNAservedbothasgenome and as catalyst. The central catalyst in an RNA world organism would have been a ribozyme that catalyzed RNA polymerizationtofacilitateself-replication.AnRNApolymeraseribozymewasdevelopedpreviouslyinthelabbutitisnot efficientenoughforself-replication. Thefactor thatlimitsits polymerizationefficiencyis itsweak sequence-independent bindingoftheprimer/templatesubstrate.HerewetestedwhetherRNApolymerizationcouldbeimprovedbyacationic argininecofactor,toimprovetheinteractionwiththesubstrate.InanRNAworld,aminoacid-nucleicacidconjugatescould havefacilitatedtheemergenceofthetranslationapparatusandthetransitiontoanRNPworld.Wechosetheaminoacid arginineforourstudybecausethisistheaminoacidmostadepttointeractwithRNA.Anargininecofactorwaspositioned at ten different sites on the ribozyme, using conjugates of arginine with short DNA or RNA oligonucleotides. However, polymerizationefficiencywasnotincreasedinanyofthetenpositions.Infiveofthetenpositionstheargininereducedor modulated polymerization efficiency, which gives insight into the substrate-binding site on the ribozyme. These results suggestthattheexistingpolymeraseribozymeisnotwellsuitedtousinganargininecofactor. Citation: Yao C, Moretti JE, Struss PE, Spall JA, Mu¨ller UF (2011) Arginine Cofactors on the Polymerase Ribozyme. PLoS ONE 6(9): e25030. doi:10.1371/ journal.pone.0025030 Editor:LennartRandau,Max-Planck-InstituteforTerrestrialMicrobiology,Germany ReceivedMay22,2011;AcceptedAugust23,2011;PublishedSeptember20,2011 Copyright:?2011Yaoetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedi