Brassica napus PHR1 Gene Encoding a MYB-Like Protein Functions in Response to Phosphate Starvation 英文参考文献.docVIP
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Brassica napus PHR1 Gene Encoding a MYB-Like Protein Functions in Response to Phosphate Starvation 英文参考文献
BrassicanapusPHR1GeneEncodingaMYB-LikeProtein
FunctionsinResponsetoPhosphateStarvation
FengRen,Qian-QianGuo,Li-LiChang,LiangChen,Cai-ZhiZhao,HuiZhong,Xue-BaoLi*
HubeiKeyLaboratoryofGeneticRegulationandIntegrativeBiology,CollegeofLifeSciences,CentralChinaNormalUniversity,Wuhan,China
Abstract
Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPHR1 encoding a MYB
transcription activator was isolated from Brassica napus. The characterization of nuclear localization and transcription
activationabilitysuggestBnPHR1isatranscriptionalactivator.Thetissueexpressionandhistochemicalassayshowedthat
BnPHR1 waspredominantlyexpressed inroots andmodulatedby exogenousPiin transcriptionallevel inroots under Pi
deficiencyconditions.Furthermore,overexpressionofBnPHR1inbothArabidopsisandB.napusremarkablyenhancedthe
expression of the Pi-starvation-induced genes including ATPT2 and BnPT2 encoding the high-affinity Pi transporter.
Additionally, BnPHR1 can invivo bind the promoter sequence of ATPT2 and BnPT2 in both Arabidopsis and B. napus.
Possibly, due to the activation of ATPT2 and BnPT2, or even more high-affinity Pi transporters, the excessive Pi was
accumulated in transgenic plants, resulting in the crucially Pi toxicity to cells and subsequently retarding plant growth.
Given the data together, BnPHR1, as crucial regulator, is regulated by exogenous Pi and directly activates those genes,
whichpromotetheuptakeandhomeostasisofPiforplantgrowth.
Citation:RenF,GuoQ-Q,ChangL-L,ChenL,ZhaoC-Z,etal.(2012)BrassicanapusPHR1GeneEncodingaMYB-LikeProteinFunctionsinResponsetoPhosphate
Starvation.PLoSONE7(8):e44005.doi:10.1371/journal.pone.0044005
Editor:LuisHerrera-Estrella,CentrodeInvestigacio′nydeEstudiosAvanzadosdelIPN,Mexico
ReceivedApril21,2012;AcceptedJuly27,2012;PublishedAugust29,2012
Copyright:?2012Renetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted
use,distribution,andrepro
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