Cardiosphere-Derived Cells Improve Function in the Infarcted Rat Heart for at Least 16 Weeks – an MRI Study 英文参考文献.docVIP

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Cardiosphere-Derived Cells Improve Function in the Infarcted Rat Heart for at Least 16 Weeks – an MRI Study 英文参考文献.doc

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Cardiosphere-Derived Cells Improve Function in the Infarcted Rat Heart for at Least 16 Weeks – an MRI Study 英文参考文献

Cardiosphere-DerivedCellsImproveFunctioninthe InfarctedRatHeartforatLeast16Weeks–anMRIStudy CarolynA.Carr1*,DanielJ.Stuckey1,2,JunJieTan1,3,SuatChengTan1,RenataS.M.Gomes1 ,Patrizia Camelliti1,2,ElisaMessina4,AlessandroGiacomello4,GeorginaM.Ellison5,KieranClarke1 1DepartmentofPhysiology,AnatomyandGenetics,UniversityofOxford,Oxford,UnitedKingdom,2NationalHeartandLungInstitute,ImperialCollege,London,United Kingdom,3ClusterofCardiovascularScience,AdvancedMedicalandDentalInstitute,UniversitiSainsMalaysia,Penang,Malaysia,4DepartmentofExperimentalMedicine, Cenci-BolognettiFoundation,PasteurInstitute,UniversityLaSapienza,Rome,Italy,5StemCellandMolecularPhysiologyLaboratory,LiverpoolJMUniversity,Liverpool, UnitedKingdom Abstract Aims:Endogenouscardiacprogenitorcells,expandedfromexplantsviacardiosphereformation,presentapromisingcell sourcetoprevent heart failurefollowing myocardial infarction. Hereweusedcine-magnetic resonance imaging(MRI)to trackadministeredcardiosphere-derivedcells(CDCs)andtomeasurechangesincardiacfunctionoverfourmonthsinthe infarctedratheart. Methods and Results: CDCs, cultured from neonatal rat heart, comprised a heterogeneous population including cells expressingthemesenchymalmarkersCD90andCD105,thestemcellmarkerc-kitandthepluripotencymarkersSox2,Oct3/ 4andKlf-4.CDCs(26106)expressinggreenfluorescentprotein(GFP+)werelabelledwithfluorescentmicron-sizedparticles of iron oxide (MPIO). Labelled cells were administered to the infarcted rat hearts (n=7) by intramyocardial injection immediatelyfollowingreperfusion,thenbysystemicinfusion(4610 )2dayslater.Acontrolgroup(n=7)wasadministered cellmedium.MRhypointensitiescausedbytheMPIOsweredetectedatalltimesandGFP+cellscontainingMPIOparticles wereidentifiedintissueslicesat16weeks.Attwodaysafterinfarction,cardiacfunctionwassimilarbetweengroups.By6 weeks, ejection fractions incontrol hearts hadsignificantly decreased (4762%), but this wasnot evidentin CDC-treated hearts(5663%).Thesignificantlyhigherejectionfractio