大孔树脂纯化废次烟叶中烟草多酚的工艺.pdfVIP

学兔兔 化 工 进 展 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2012年第3 1卷第12期 大孑L树脂纯化废次烟叶中烟草多酚的工艺 张献忠，钟烈洲，黄海智，叶兴乾 (浙江大学生物系统工程与食品科学学院，浙江 杭州 310058) 摘 要：通过吸附和解吸附实验，从 6种大孔树脂中筛选出适于分离纯化废次烟叶中烟草多酚的大孔树脂，并 确定该大孔树脂纯化工艺参数。结果显示，D101大孔树脂能够较好的富集纯化废次烟叶中的烟草多酚，当提取 液浓度为2．5 mg／mL，pH值为4．0，上样液流速为2．0 mL／min，洗脱液为80％乙醇溶液，洗脱液流速为2．0 mL／min 时，纯化效果最好，烟草多酚的动态回收率为70．37％。 关键词：大孔树脂；废次烟叶；多酚；_T-艺 中图分类号：TS49 文献标志码：A 文章编号：1000—6613(2012)12—2626—06 Purification of polyphenol from discarded tobacco leaves by macroporous adsorption resin ZHANGXianzhong，ZHONG Liezhou．HUNAG Haizhi YEXingqian (School ofBiosystems Engineering and Food Science，Zhejiang University,Hangzhou 3 10058，Zhejiang，China) Abstract：Absorption and desorption experiments were performed to screen the suitable resin from 6 types of macroporous absorbent resins for the purification of tobacco polyphenol from discarded tobacco leaves．Results indicate that macroporous resin D101 possesses the strongest absorption and desorption capacity．The optimal absorption and desorption condition of macroporous resin D I O 1 is the concentration of feeding solution 2．5 rag／mE，pH value 4．0，feeding rate 2．0 mL／min，elute ethanol concentration 80％，elution rate 2．0 mL／min．The dynamic recovery of tobacco polyphenol was 70．37％ under the optimal condition． Key words：macroporous absorbent resin；discarded tobacco leaves；polyphenol；technology 植物多酚已被证明具有抗氧化、预防衰老和心 新型分离材料，具有吸附量大、选择性好、吸附迅 血管疾病等多种生物活性，目前，关于植物多酚的 速、再生简单等优点，已被广泛应用于植物多酚类 研究得到科学家越来越多的