手性化合物酶法制备中辅酶再生体系的构建与应用进展.pdfVIP

学兔兔 化 工 进 展 ·l826· CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2014年第33卷第7期 手性化合物酶法制备中辅酶再生体系的构建与应用进展 彭益强，张曙伟 (华侨大学工业生物技术福建省高等学校重点实验室，福建 厦门361021) 摘要：辅酶的再生与反复利用对氧化还原型生物催化剂在手性化合物中的生产具有重要作用。辅酶的酶催化法 再生具有高效、专一的特点，根据其催化环境可分为单细胞内辅酶再生体系、双细胞耦合体系及胞外酶耦合体 系。本文详细阐述了酶法再生中各种辅酶再生体系的构建方法，特别指出了辅酶再生体系使用中可能遇到的问 题并给出了解决方法，如利用离子液体解决有机底物低水溶性的问题，利用共价固定方法解决小分子辅酶的留 存问题，无细胞抽提液中内源性辅酶再生酶的利用等。除此之外，对每种辅酶再生方法与体系在工业催化中应 用的优势与局限性作了评述，特别关注了辅酶再生体系反复多批次利用的问题。 关键词：手性化合物；辅酶再生体系；酶耦合反应；细胞耦合；内源性辅酶再生酶 中图分类号：Q 552 文献标志码：A 文章编号：1000—6613(2014)07—1826—06 DoI：1O．3969~．issn．1000—6613-20l4．07．029 Progress of cofactor regeneration system construction in chiral compound production by enzymatic catalysis PENG Yiqiang，ZHANG Shuwei (Provincial Key Laboratory ofIndustrial Biotechnology，Huaqiao University，Xiamen 361021，Fujian，China) Abstract：Cofactor regeneration and repeated utilization showed important effect on the preparation of chiral compound by the redox biocatalysts．Cofactor regenerated by enzymatic catalysis had high efficiency and specificity，such cofactor regenerated system include single cell in vivo cofactor regenerated system，double cell coupling system and in vitro enzyme coupling system due to their catalysis circumstance．The construct methods of different cofactor regenerated system were presented particularly in this paper~The possible issues in practical application of these cofactor regenerated system were especially pointed out and the resolve methods were afforded，such as the solution of low water-soluble organic