Development of a Novel Fluorophore for Real-Time Biomonitoring System 英文参考文献.docVIP

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Development of a Novel Fluorophore for Real-Time Biomonitoring System 英文参考文献.doc

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Development of a Novel Fluorophore for Real-Time Biomonitoring System 英文参考文献

DevelopmentofaNovelFluorophoreforReal-Time BiomonitoringSystem Hyun-OkSong1,BinnaLee1,RamPrasadBhusal2,ByounghunPark3,KyoungsikYu3,Chom-KyuChong4, PyoYunCho1¤,SungYeonKim2,HakSungKim2*,HyunPark1* 1Zoonosis Research Center, Department of Infection Biology, Wonkwang University College of Medicine, Iksan, Jeonbuk, Republic of Korea, 2College of Pharmacy, InstituteofPharmaceuticalResearchandDevelopment,WonkwangUniversity,Iksan,Jeonbuk,RepublicofKorea,3DepartmentofElectricalEngineering,KoreaAdvanced Institute of Science and Technology, Daejeon, Republic of Korea, 4Department of Biochemistry, Division of Life Science, Chungbuk National University, Cheongju, Chungbuk,RepublicofKorea Abstract Rapid in-field diagnosis is very important to prevent the outbreak of various infectious and contagious diseases. Highly sensitiveandquantitativedetectionofdiseasescanbeperformedusingfluorescentimmunochemicalassaywithspecific antigen-antibody binding and a good quality fluorophore. This can lead to the development of a small, portable, quantitativebiosensortotransmitdiagnosticresultstoacontrolcenterinordertosystematicallypreventdiseaseoutbreaks. Inthisstudy,wedevelopedanovelfluorophore,coumarin-deriveddendrimer,withhighemissionintensity,strongsignal brightness, and high photostability. It is easily coupled with biomolecules and emits strong and stable fluorescence at 590nmwithexcitationat455nm.Applicationtofluorescentimmunochromatographictest(FICT)showedthatthenovel coumarin-deriveddendrimerbioconjugatecoulddetectantigensatamountaslowas0.1ng.Theclinicalresultsandthe spectralcharacteristicsofthenovelcoumarin-deriveddendrimeropen,forthefirsttime,thepossibilityofdevelopingacost/ energyefficientLED-basedportablequantitativebiosensorforpoint-of-care(POC)diseasediagnosis,whichcanpermitreal timemonitoring(U-healthcaresystem)byadiseasecontrolcenter. Citation: Song H-O, Lee B, Bhusal RP, Park B, Yu K, etal. (2012) Development of a Novel Fluorophorefor Real-Time Biomonitoring System. PLoS ONE 7(