Dia2 Controls Transcription by Mediating Assembly of the RSC Complex 英文参考文献.docVIP

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Dia2 Controls Transcription by Mediating Assembly of the RSC Complex 英文参考文献.doc

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Dia2 Controls Transcription by Mediating Assembly of the RSC Complex 英文参考文献

Dia2ControlsTranscriptionbyMediatingAssemblyof theRSCComplex EdwardJ.Andress1,RomanHolic1,MariolaJ.Edelmann2,BenediktM.Kessler2,VeronicaP.C.C.Yu1,3* 1EukaryoticChromatinDynamicsGroup,MRCClinicalSciencesCentre,ImperialCollegeHammersmithCampus,London,UnitedKingdom,2UbiquitinProteolysisGroup, Central Proteomics Facility, Nuffield Department of Clinical Medicine, Centre for Cellular and Molecular Physiology, Oxford University, Oxford, United Kingdom, 3DepartmentofMedicalandMolecularGenetics,King’sCollegeLondonSchoolofMedicine,Guy’sHospital,London,UnitedKingdom Abstract Background:Dia2isanF-boxproteinfoundinthebuddingyeast,S.cerevisiae.TogetherwithSkp1andCul1,Dia2formsthe substrate-determining part of an E3 ubiquitin ligase complex, otherwise known as the SCF. Dia2 has previously been implicatedinthecontrolofreplicationandgenomestabilityviaitsinteractionwiththereplisomeprogressioncomplex. PrincipalFindings:WeidentifiedcomponentsoftheRSCchromatinremodellingcomplexasgeneticinteractorswithDia2, suggesting an additional role for Dia2 in the regulation of transcription. We show that Dia2 is involved in controlling assemblyoftheRSCcomplex.RSCbelongstoagroupofATP-dependentnucleosome-remodellingcomplexesthatcontrols therepositioningofnucleosomes.TheRSCcomplexisexpressedabundantlyandits17subunitsarerecruitedtochromatin inresponsetobothtranscriptionactivationandrepression.IntheabsenceofDia2,RSC-mediatedtranscriptionregulation wasimpaired,withconcomitantabnormalitiesinnucleosomepositioning. Conclusions:OurfindingsimplythatDia2isrequiredforthecorrectassemblyandfunctionoftheRSCcomplex.Dia2,by controlling the RSC chromatin remodeller, fine-tunes transcription by controlling nucleosome positioning during transcriptionalactivationandrepression. Citation:AndressEJ,HolicR,EdelmannMJ,KesslerBM,YuVPCC(2011)Dia2ControlsTranscriptionbyMediatingAssemblyoftheRSCComplex.PLoSONE6(6): e21172.doi:10.1371/journal.pone.0021172 Editor:Dong-YanJin,UniversityofHongKong,HongKong ReceivedDecember23,201