Distinct Distribution of Ectopically Expressed Histone Variants H2A.Bbd and MacroH2A in Open and Closed Chromatin Domains 英文参考文献.docVIP

下载本文档

4
0
约5.62万字
约 14页
2017-05-11 发布于上海
举报
版权申诉

Distinct Distribution of Ectopically Expressed Histone Variants H2A.Bbd and MacroH2A in Open and Closed Chromatin Domains 英文参考文献.doc

1、本文档共14页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Distinct Distribution of Ectopically Expressed Histone Variants H2A.Bbd and MacroH2A in Open and Closed Chromatin Domains 英文参考文献

DistinctDistributionofEctopicallyExpressedHistone VariantsH2A.BbdandMacroH2AinOpenandClosed ChromatinDomains ElenaS.Ioudinkova1,2,3.,AnaBarat2,4.,AndreyPichugin2,3,ElenaMarkova1,2,3,IlyaSklyar1,2,3 , IrynaPirozhkova2,3,ChloeRobin2,3,MarcLipinski2,3,VasilyOgryzko2,3,YegorS.Vassetzky2,3*, SergeyV.Razin1,3 1InstituteofGeneBiology,RussianAcademyofSciences,Moscow,Russia,2CNRSUMR8126,Univ.Paris-Sud11,Institutdecance′rologieGustaveRoussy,Villejuif,France, 3LIA1066,LaboratoireFranco-Russederecherchesenoncologie,Villejuif,France,4TheCentreforScientificComputingComplexSystemsModelling(SCI-SYM),School ofComputing,DublinCityUniversity,Dublin,Ireland Abstract Background:Itbecomesincreasinglyevidentthatnuclesomesarefarfrombeingidenticaltoeachother.Thisnucleosome diversityisduepartiallytotheexistenceofhistonevariantsencodedbyseparategenes.Amongtheknownhistonevariants thelesscharacterizedareH2A.BbdanddifferentformsofmacroH2A.ThisisespeciallytrueinthecaseofH2A.Bbdasthere are still no commercially available antibodies specific to H2A.Bbd that can be used for chromatin immunoprecipitation (ChIP). Methods: We have generated HeLa S3 cell lines stably expressing epitope-tagged versions of macroH2A1.1, H2A.Bbd or canonicalH2AandanalyzedgenomicdistributionofthetaggedhistonesusingChIP-on-chiptechnique. Results:ThepresenceofhistoneH2AvariantsmacroH2A1.1andH2A.Bbdhasbeenanalyzedinthechromatinofseveral segmentsofhumanchromosomes11,16andXthathavebeenchosenfortheirdifferentgenedensitiesandchromatin status. Chromatin immunoprecipitation (ChIP) followed by hybridization with custom NimbleGene genomic microarrays demonstrated that in open chromatin domains containing tissue-specific along with housekeeping genes, the H2A.Bbd variantwaspreferentiallyassociatedwiththebodyofasubsetoftranscribedgenes.ThemacroH2A1.1variantwasvirtually absent from some genes and underrepresented in others. In contrast, in closed chromatin domains which contain only tissue-specific genes inactive in HeLa S3 cells, both