Diversity Analysis of Streptomycetes and Associated Phosphotranspherase Genes in Soil 英文参考文献.docVIP

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Diversity Analysis of Streptomycetes and Associated Phosphotranspherase Genes in Soil 英文参考文献.doc

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Diversity Analysis of Streptomycetes and Associated Phosphotranspherase Genes in Soil 英文参考文献

DiversityAnalysisofStreptomycetesandAssociated PhosphotranspheraseGenesinSoil ParisLaskaris*,TakuyaSekine,ElizabethM.H.Wellington SchoolofLifeSciences,UniversityofWarwick,Coventry,UnitedKingdom Abstract AnattemptwasmadetoverifytheobservationthatStreptomycesgriseuswasprevalentinsoilbasedonisolationwork.A genus-specificPCRwasdevelopedforStreptomycesbasedonthehousekeepinggeneatpDandusedtoinvestigatespecies diversity within selected soils. The presence of S. griseus was investigated to determine coexistence of resistance-only streptomycinphosphotransferase(strA)inthesamesoilasstreptomycinproducers.TwoadditionalPCR-basedassayswere developed; one specific for strA in association with production, the other for more diverse strA and other related phosphotranferases.BoththeS.griseusatpDandstrAgeneswerebelowthePCRdetectionlimitinallsoilsexamined.A number of more diverse phosphotransferase genes were amplified, a minority of which may be associated with streptomycinproduction.WeconcludethatneitherstreptomycinproducersnorS.griseusareprevalentinthefreshorchitin andstarch-amendedsoilsexamined(lessthan0.1%ofsoilactinobacteria).Oneofthesoilsiteshadreceivedplantomycin (activeingredient:streptomycin)anddiversitystudiessuggestedthatthisalteredthestreptomycetepopulationspresentin thesoil. Citation:LaskarisP,SekineT,WellingtonEMH(2012)DiversityAnalysisofStreptomycetesandAssociatedPhosphotranspheraseGenesinSoil.PLoSONE7(4): e35756.doi:10.1371/journal.pone.0035756 Editor:AlfredoHerrera-Estrella,Cinvestav,Mexico ReceivedSeptember23,2011;AcceptedMarch26,2012;PublishedApril23,2012 Copyright: ? 2012 Laskaris et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisprojectwassupportedbygrantsfromtheUnitedKingdomBiotechnologyandBiologicalSciencesResearchCouncil(grantreference:BBSRCBB/ F003498/1)andtheEuropeanUnio