Diversity of 23S rRNA Genes within Individual Prokaryotic Genomes 英文参考文献.docVIP

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Diversity of 23S rRNA Genes within Individual Prokaryotic Genomes 英文参考文献.doc

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Diversity of 23S rRNA Genes within Individual Prokaryotic Genomes 英文参考文献

Diversityof23SrRNAGeneswithinIndividual ProkaryoticGenomes AnnaPei1,CarlosW.Nossa2,PoojaChokshi3,MartinJ.Blaser2,4,5,LiyingYang2,DavidM.Rosmarin2, ZhihengPei2,5,6 * 1WashingtonUniversity,CollegeofArtsandSciences,St.Louis,Missouri,UnitedStatesofAmerica,2DepartmentofMedicine,NewYorkUniversitySchoolofMedicine, New York, New York, United States of America, 3Tufts University College of Arts and Sciences, Medford, Massachusetts, United States of America, 4Department of Microbiology,NewYorkUniversitySchoolofMedicine,NewYork,NewYork,UnitedStatesofAmerica,5DepartmentofVeteransAffairs,NewYorkHarborHealthcare System, New York, New York, United States of America, 6Department of Pathology, New York University School of Medicine, New York, New York, United States of America Abstract Background: The concept of ribosomal constraints on rRNA genes is deduced primarily based on the comparison of consensus rRNA sequences between closely related species, but recent advances in whole-genome sequencing allow evaluationofthisconceptwithinorganismswithmultiplerRNAoperons. Methodology/Principal Findings: Using the 23S rRNA gene as an example, we analyzed the diversity among individual rRNAgeneswithinagenome.Of184prokaryoticspeciescontainingmultiple23SrRNAgenes,diversitywasobservedin113 (61.4%) genomes (mean 0.40%, range 0.01%–4.04%). Significant (1.17%–4.04%) intragenomic variation was found in 8 species.In5ofthe8species,thediversityintheprimarystructurehadonlyminimaleffectonthesecondarystructure(stem versus loop transition). In the remaining 3 species, the diversity significantly altered local secondary structure, but the alterationappearsminimizedthroughcomplexrearrangement.Interveningsequences(IVS),rangingbetween9and1471nt insize,werefoundin7species.IVSinDeinococcusradioduransandNostocsp.encodetransposases.T.tengcongensiswasthe onlyspeciesinwhichintragenomicdiversity.3%wasobservedamong4paralogous23SrRNAgenes. Conclusions/Significance: These findings indicate tight ribosomal constraints