Diversity and Relationships of Eggplants from Three Geographically Distant Secondary Centers of Diversity 英文参考文献.docVIP

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Diversity and Relationships of Eggplants from Three Geographically Distant Secondary Centers of Diversity 英文参考文献.doc

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Diversity and Relationships of Eggplants from Three Geographically Distant Secondary Centers of Diversity 英文参考文献

DiversityandRelationshipsofEggplantsfromThree GeographicallyDistantSecondaryCentersofDiversity MariaHurtado1,SantiagoVilanova1,MariolaPlazas1,PietroGramazio1,H.HemalFonseka2, RamyaFonseka3,JaimeProhens1* 1InstitutodeConservacio′nyMejoradelaAgrodiversidadValenciana,UniversitatPolite`cnicadeVale`ncia,Valencia,Spain,2HorticulturalCropResearchandDevelopment Institute,Gannoruwa,SriLanka,3DepartmentofCropScience,FacultyofAgriculture,UniversityofPeradeniya,Peradeniya,SriLanka Abstract Eggplant (Solanum melongena L.) was domesticated in the Indo-Birmanian region, which is also the primary center of diversityforthiscrop.Fromthereeggplantspreadtootherregions,anddiversityaccumulatedinseveralsecondarycenters ofdiversity.Wehaveassessedthediversityandrelationshipsof52accessionsofeggplantfromthreegeographicallydistant secondarycentersofdiversity(China,Spain,andSriLanka)using28morphologicaldescriptorsand12highlypolymorphic genomic SSRs. A wide variation was found for most morphological traits, and significant differences among the three centersofdiversityweredetectedfor22ofthesetraits.ThePCAanalysisshowedthateggplantsfromthethreeoriginswere morphologically differentiated, and accessions from each of the three secondary centers of diversity presented a typical combination of morphological characteristics. In this respect, discriminant analysis showed that accessions could be correctlyclassifiedtotheiroriginusingonlysixtraits.TheSSRcharacterizationidentified110allelesandallowedobtaininga uniquegeneticfingerprintforeachaccession.Manyalleleswerefoundtobeprivatetoeachorigin,butnouniversalalleles werefoundforanyoftheorigins.ThePCAanalysisshowedthatthegeneticdifferentiationamongoriginswaslessclear than for morphological traits, although the analysis of the population structure shows that accessions mostly group according to the origin, but also provides evidence of migration among the three secondary centers of diversity. The geneticdiversity(HT)withineachoriginwashigh,rangingbetweenHT=