Dynamic Expression of the Translational Machinery during Bacillus subtilis Life Cycle at a Single Cell Level 英文参考文献.docVIP

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Dynamic Expression of the Translational Machinery during Bacillus subtilis Life Cycle at a Single Cell Level 英文参考文献.doc

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Dynamic Expression of the Translational Machinery during Bacillus subtilis Life Cycle at a Single Cell Level 英文参考文献

DynamicExpressionoftheTranslationalMachinery duringBacillussubtilisLifeCycleataSingleCellLevel AlexRosenberg1,LiorSinai1,YoavSmith2,SigalBen-Yehuda1* 1DepartmentofMicrobiologyandMolecularGenetics,InstituteforMedicalResearchIsrael-Canada(IMRIC),TheHebrewUniversity,Hadassah-MedicalSchool,TheHebrew UniversityofJerusalem,Jerusalem,Israel,2GenomicDataAnalysisUnit,TheHebrewUniversity-HadassahMedicalSchool,TheHebrewUniversityofJerusalem,Jerusalem, Israel Abstract Theabilityofbacteriatoresponsivelyregulatetheexpressionoftranslationcomponentsiscrucialforrapidadaptationto fluctuating environments. Utilizing Bacillus subtilis (B. subtilis) as a model organism, we followed the dynamics of the translational machinery at a single cell resolution during growth and differentiation. By comprehensive monitoring the activityofthemajorrrnpromotersandribosomalproteinproduction,werevealeddiversedynamicsbetweencellsgrownin rich and poor medium, with the most prominent dissimilarities exhibited during deep stationary phase. Further, the variabilitypatternoftranslationalactivityvariedamongthecells,beingaffectedbynutrientavailability.Wehavemonitored forthefirsttimetranslationaldynamicsduringthedevelopmentalprocessofsporulationwithinthetwodistinctcellular compartments of forespore and mother-cell. Our study uncovers a transient forespore specific increase in expression of translationalcomponents.Finally,thecontributionofeachrrnpromoterthroughoutthebacteriumlifecyclewasfoundto berelativelyconstant,implyingthatdifferentialexpressionisnotthemainpurposefortheexistenceofmultiplerrngenes. Instead,weproposethatcoordinationoftherrnoperonsservesasastrategytorapidlyfinetunetranslationalactivitiesin asynchronizedfashiontoachieveanoptimaltranslationlevelforagivencondition. Citation:RosenbergA,SinaiL,SmithY,Ben-YehudaS(2012)DynamicExpressionoftheTranslationalMachineryduringBacillussubtilisLifeCycleataSingleCell Level.PLoSONE7(7):e41921.doi:10.1371/journal.pone.0041921 Editor:ChristopheHerman,BaylorColle