Dynamic Modulation of DNA Replication and Gene Transcription in Deep-Sea Filamentous Phage SW1 in Response to Changes of Host Growth and Temperature 英文参考文献.docVIP

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Dynamic Modulation of DNA Replication and Gene Transcription in Deep-Sea Filamentous Phage SW1 in Response to Changes of Host Growth and Temperature 英文参考文献.doc

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Dynamic Modulation of DNA Replication and Gene Transcription in Deep-Sea Filamentous Phage SW1 in Response to Changes of Host Growth and Temperature 英文参考文献

DynamicModulationofDNAReplicationandGene TranscriptioninDeep-SeaFilamentousPhageSW1in ResponsetoChangesofHostGrowthandTemperature HuahuaJian1,2,JunXu2,XiangXiao2,FengpingWang2,3* 1SchoolofLifeSciences,XiamenUniversity,Xiamen,China,2StateKeyLaboratoryofMicrobialMetabolismandSchoolofLifeSciencesandBiotechnology,StateKey LaboratoryofOceanEngineering,ShanghaiJiaoTongUniversity,Shanghai,PRChina,3KeyLaboratoryofSystemsBiomedicine,MinistryofEducation,ShanghaiJiaoTong University,Shanghai,PRChina Abstract Littleisknownabouttheresponseofdeep-seavirusandtheirrelationshipwiththeirhosttowardsenvironmentalchange. Althoughvirusesarethoughttoplaykeyrolesinthedeep-seaecologicalevolutionandbiogeochemicalcycling,theseroles areyettobedefined.Thisstudyaimstodelineatetherelationshipbetweenadeep-seafilamentousphageSW1anditshost Shewanellapiezotolerans(S.piezotolerans)WP3,andtheirresponsetowardstemperaturechange.ThecopynumberofSW1’s replicativeform(RF-)DNAandsingle-stranded(ss-)DNAalongthedifferentgrowthphasesofWP3werequantifiedat20uC and4uC,respectively.ThecopynumberofSW1RF-DNAwasfoundtobetemperatureandgrowthphase-dependent,while thessDNAofSW1wasonlyproducedat4uC.Thisisthefirstreportshowinglow-temperaturedependenceofphageDNA replication.ThetranscriptionofSW1keygenesfpsAandfpsRwerealsofoundtobeinducedatlowtemperatureduringall themonitoredgrowthperiodsofWP3.Additionally,thetranscriptionofSW1wasfoundtobeinducedbycold-shockwhile itsDNAreplicationwasnotchanged.OurdatademonstratesadynamicchangeofvirusDNAreplicationandtranscriptionin accordancewithhostgrowth,andthelowtemperatureadaptedmechanismsforSW1activitiesinthedeepsea.Thislow temperatureadapteddeep-seavirus-bacteriumsystemcouldserveasanidealmodeltofurtherstudythemechanismand relationshipofdeep-seavirus-bacteriaecosystems. Citation:JianH,XuJ,XiaoX,WangF(2012)DynamicModulationofDNAReplicationandGeneTranscriptioninDeep-SeaFilamentousPhageSW1inResponse toChangesofHostGrowthandTemperature.PLoSONE7(8):e41578.doi:10.1371/journal.pone.0041578 Edi