Effects of Dimethyl Sulfoxide in Cholesterol-Containing Lipid Membranes A Comparative Study of Experiments In Silico and with Cells 英文参考文献.docVIP

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Effects of Dimethyl Sulfoxide in Cholesterol-Containing Lipid Membranes A Comparative Study of Experiments In Silico and with Cells 英文参考文献.doc

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Effects of Dimethyl Sulfoxide in Cholesterol-Containing Lipid Membranes A Comparative Study of Experiments In Silico and with Cells 英文参考文献

EffectsofDimethylSulfoxideinCholesterol-Containing LipidMembranes:AComparativeStudyofExperiments InSilicoandwithCells Marie-Ame′liedeMe′norval1,2,3,LluisM.Mir1,2,3,M.LauraFerna′ndez4,5,RamonReigada6* 1Universite′ Paris-Sud, Laboratoire de Vectorologie et The′rapeutiques Anticance′reuses, UMR 8203, Orsay, France, 2CNRS, Orsay, Laboratoire de Vectorologie et The′rapeutiquesAnticance′reuses,UMR8203,Orsay,France,3InstitutGustaveRoussy,LaboratoiredeVectorologieetThe′rapeutiquesAnticance′reuses,UMR8203,Villejuif, France, 4Laboratorio de Sistemas Complejos, Departamento de Computacio′n, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina, 5Consejo Nacional de Investigaciones Cient?′ficas y Te′cnicas, Buenos Aires, Argentina, 6Department de Qu?′mica F?′sica and Institut de Qu?′mica Teo`rica i Computacional,UniversitatdeBarcelona,Barcelona,Spain Abstract Dimethylsulfoxide(DMSO)hasbeenknowntoenhancecellmembranepermeabilityofdrugsorDNA.Moleculardynamics (MD) simulations with single-component lipid bilayers predicted the existence of three regimes of action of DMSO: membraneloosening,poreformationandbilayercollapse.Weshowherethatthesemodesofactionarealsoreproducedin thepresenceofcholesterolinthebilayer,andweprovideadescriptionattheatomicdetailoftheDMSO-mediatedprocess of pore formation in cholesterol-containing lipid membranes. We also successfully explore the applicability of DMSO to 2+ promote plasma membrane permeability to water, calcium ions (Ca ) and Yo-Pro-1 iodide (Yo-Pro-1) in living cell membranes.Theexperimentalresultsoncellsinculturecanbeeasilyexplainedaccordingtothethreeexpectedregimes:in thepresenceoflowdosesofDMSO,themembraneofthecellsexhibitsundulationsbutnopermeabilityincreasecanbe detected, while at intermediate DMSO concentrations cells are permeabilized to water and calcium but not to larger molecules as Yo-Pro-1. These two behaviors can be associated to the MD-predicted consequences of the effects of the DMSOatlo