Epigenomic Consequences of Immortalized Plant Cell Suspension Culture 英文参考文献.docVIP

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Epigenomic Consequences of Immortalized Plant Cell Suspension Culture 英文参考文献.doc

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Epigenomic Consequences of Immortalized Plant Cell Suspension Culture 英文参考文献

o PL SBIOLOGY EpigenomicConsequencesofImmortalized PlantCellSuspensionCulture Milos Tanurdzic1,Matthew W. Vaughn1,Hongmei Jiang2,Tae-Jin Lee3,R.Keith Slotkin1,Bryon Sosinski3, William F.Thompson4,R.W. Doerge5,Robert A.Martienssen1 1ColdSpringHarborLaboratory,ColdSpringHarbor,NewYork,UnitedStatesofAmerica,2DepartmentofStatistics,NorthwesternUniversity,Evanston,Illinois,United StatesofAmerica,3DepartmentofHorticulturalScience,NorthCarolinaStateUniversity,Raleigh,NorthCarolina,UnitedStatesofAmerica,4DepartmentsofPlantBiology, Genetics,andCropScience,NorthCarolinaStateUniversity,Raleigh,NorthCarolina,UnitedStatesofAmerica,5DepartmentsofStatisticsandAgronomy,PurdueUniversity, WestLafayette,Indiana,UnitedStatesofAmerica Plant cells grown in culture exhibit genetic and epigenetic instability. Using a combination of chromatin immunoprecipitation and DNA methylation profiling on tiling microarrays, we have mapped the location and abundanceofhistoneandDNAmodificationsinacontinuouslyproliferating,dedifferentiatedcellsuspensionculture ofArabidopsis.Wehavefoundthateuchromatinbecomeshypermethylatedincultureandthatasmallpercentageof the hypermethylated genes become associated with heterochromatic marks. In contrast, the heterochromatin undergoes dramatic and very precise DNA hypomethylation with transcriptional activation of specific transposable elements(TEs)inculture.HighthroughputsequencingofsmallinterferingRNA(siRNA)revealedthatTEsactivatedin culture have increased levels of 21-nucleotide (nt) siRNA, sometimes at the expense of the 24-nt siRNA class. In contrast,TEsthatremainsilent,whichmatchthepredominant24-ntsiRNAclass,donotchangesignificantlyintheir siRNAprofiles.TheseresultsimplicateRNAinterferenceandchromatinmodificationinepigeneticrestructuringofthe genomefollowingtheactivationofTEsinimmortalizedcellculture. Citation:TanurdzicM,VaughnMW,JiangH,LeeTJ,SlotkinRK,etal.(2008)Epigenomicconsequencesofimmortalizedplantcellsuspensionculture.PLoSBiol6(12):e302. doi:10.137