Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection 英文参考文献.docVIP

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Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection 英文参考文献.doc

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Establishment of Fruit Bat Cells (Rousettus aegyptiacus) as a Model System for the Investigation of Filoviral Infection 英文参考文献

EstablishmentofFruitBatCells(Rousettusaegyptiacus) asaModelSystemfortheInvestigationofFiloviral Infection VerenaKra¨hling1,OlgaDolnik1,LarissaKolesnikova1,JonasSchmidt-Chanasit2,IngoJordan3 ,Volker Sandig3,StephanGu¨nther2,StephanBecker1* 1Institut fu¨r Virologie, Philipps-Universita¨t Marburg, Marburg, Germany, 2Institut fu¨r Virologie, Bernhard-Nocht-Institut fu¨r Tropenmedizin, Hamburg, Germany, 3ProBioGenAG,Berlin,Germany Abstract Background:ThefruitbatspeciesRousettusaegyptiacuswasidentifiedasapotentialreservoirforthehighlypathogenic filovirusMarburgvirus.Toestablishabasisforamolecularunderstandingofthebiologyoffilovirusesinthereservoirhost, wehaveadaptedasetofmoleculartoolsforinvestigationoffilovirusreplicationinarecentlydevelopedcellline,R06E, derivedfromthespeciesRousettusaegyptiacus. Methodology/PrincipalFindings:UponinfectionwithEbolaorMarburgviruses,R06Ecellsproducedviraltiterscomparable toVeroE6cells,asshownbyTCID50analysis.Electronmicroscopicanalysisofinfectedcellsrevealedmorphologicalsignsof filovirusinfectionasdescribedforhuman-andmonkey-derivedcelllines.UsingR06Ecells,wedetectedanunusuallyhigh amountofintracellularviralproteins,whichcorrelatedwiththeaccumulationofhighnumbersoffiloviralnucleocapsidsin thecytoplasm.WeestablishedprotocolstoproduceMarburginfectiousvirus-likeparticlesfromR06Ecells,whichwerethen usedtoinfectna?¨vetargetcellstoinvestigateprimarytranscription.Thiswasnotpossiblewithothercelllinespreviously tested.Moreover,weestablishedprotocolstoreliablyrescuerecombinantMarburgvirusesfromR06Ecells. Conclusion/Significance:ThesedataindicatedthatR06Ecellsarehighlysuitabletoinvestigatethebiologyoffilovirusesin cellsderivedfromtheirpresumedreservoir. Citation:Kra¨hlingV,DolnikO,KolesnikovaL,Schmidt-ChanasitJ,JordanI,etal.(2010)EstablishmentofFruitBatCells(Rousettusaegyptiacus)asaModelSystem fortheInvestigationofFiloviralInfection.PLoSNeglTropDis4(8):e802.doi:10.1371/journal.pntd.0000802 Editor:ThomasWilliamGeisbert,UniversityofTexasMedicalBranc