Expression and Trans-Specific Polymorphism of Self-Incompatibility RNases in Coffea (Rubiaceae) 英文参考文献.docVIP

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Expression and Trans-Specific Polymorphism of Self-Incompatibility RNases in Coffea (Rubiaceae) 英文参考文献.doc

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Expression and Trans-Specific Polymorphism of Self-Incompatibility RNases in Coffea (Rubiaceae) 英文参考文献

ExpressionandTrans-SpecificPolymorphismofSelf- IncompatibilityRNasesinCoffea(Rubiaceae) MichaelD.Nowak1*¤,AaronP.Davis2,Franc?oisAnthony3,AnneD.Yoder1 1DepartmentofBiology,DukeUniversity,Durham,NorthCarolina,UnitedStatesofAmerica,2RoyalBotanicGardens,Kew,Richmond,Surrey,UnitedKingdom,3Institut deRecherchepourleDe′veloppement,centredeMontpellier,Unite′ MixtedeRecherche‘‘Re′sistance desPlantesauxBioagresseurs’’,Montpellier,France Abstract Self-incompatibility(SI)iswidespreadintheangiosperms,butidentifyingthebiochemicalcomponentsofSImechanisms hasproventobedifficultinmostlineages.Coffea(coffee;Rubiaceae)isagenusofold-worldtropicalunderstorytreesin whichthevastmajorityofdiploidspeciesutilizeamechanismofgametophyticself-incompatibility(GSI).TheS-RNaseGSI systemwasoneofthefirstSImechanismstobebiochemicallycharacterized,andlikelyrepresentstheancestralEudicot conditionasevidencedbyitsfunctionalcharacterizationinbothasterid(Solanaceae,Plantaginaceae)androsid(Rosaceae) lineages.TheS-RNaseGSImechanismemploystheactivityofclassIIIRNaseT2proteinstoterminatethegrowthof‘‘self’’ pollentubes.Here,weinvestigatethemechanismofCoffeaGSIandspecificallyexaminethepotentialforhomologytoS- RNase GSI by sequencing class III RNase T2 genes in populations of 14 African and Madagascan Coffea species and the closely related self-compatible species Psilanthus ebracteolatus. Phylogenetic analyses of these sequences aligned to a diverse sample of plant RNase T2 genes show that the Coffea genome contains at least three class III RNase T2 genes. Patternsoftissue-specificgeneexpressionidentifyoneoftheseRNaseT2genesastheputativeCoffeaS-RNasegene.We showthatpopulationsofSICoffeaareremarkablypolymorphicforputativeS-RNasealleles,andexhibitapersistentpattern oftrans-specificpolymorphismcharacteristicofallS-RNasegenespreviouslyisolatedfromGSIEudicotlineages.Wethus concludethatCoffeaGSIis mostlikelyhomologoustotheclassicEudicotS-RNase system,whichwasretainedsincethe divergenceoftheRubiaceaelineag