Expression of a Novel Antimicrobial Peptide Penaeidin4-1 in Creeping Bentgrass (Agrostis stolonifera L.) Enhances Plant Fungal Disease Resistance 英文参考文献.docVIP

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Expression of a Novel Antimicrobial Peptide Penaeidin4-1 in Creeping Bentgrass (Agrostis stolonifera L.) Enhances Plant Fungal Disease Resistance 英文参考文献.doc

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Expression of a Novel Antimicrobial Peptide Penaeidin4-1 in Creeping Bentgrass (Agrostis stolonifera L.) Enhances Plant Fungal Disease Resistance 英文参考文献

ExpressionofaNovelAntimicrobialPeptidePenaeidin4- 1inCreepingBentgrass(AgrostisstoloniferaL.) EnhancesPlantFungalDiseaseResistance ManZhou,QianHu,ZhigangLi,DayongLi,Chin-FuChen¤,HongLuo* DepartmentofGeneticsandBiochemistry,ClemsonUniversity,Clemson,SouthCarolina,UnitedStatesofAmerica Abstract Background:Turfgrassspeciesareagriculturallyandeconomicallyimportantperennialcrops.Turfgrassspeciesarehighly susceptibletoawiderangeoffungalpathogens.Dollarspotandbrownpatch,twoimportantdiseasescausedbyfungal pathogens Sclerotinia homoecarpa and Rhizoctonia solani, respectively, are among the most severe turfgrass diseases. Currently,turffungaldiseasecontrolmainlyreliesonfungicidetreatments,whichraisesmanyconcernsforhumanhealth andtheenvironment.Antimicrobialpeptidesfoundinvariousorganismsplayanimportantroleininnateimmuneresponse. Methodology/PrincipalFindings:Theantimicrobialpeptide-Penaeidin4-1(Pen4-1)fromtheshrimp,Litopenaeussetiferus hasbeenreportedtopossessinvitroantifungalandantibacterialactivitiesagainstvariouseconomicallyimportantfungal andbacterialpathogens.Inthisstudy,wehavestudiedthefeasibilityofusingthisnovelpeptideforengineeringenhanced diseaseresistanceintocreepingbentgrassplants(AgrostisstoloniferaL.,cv.PennA-4).TwoDNAconstructswereprepared containing either the coding sequence of a single peptide, Pen4-1 or the DNA sequence coding for the transit signal peptide of the secreted tobacco AP24 protein translationally fused to the Pen4-1 coding sequence. A maize ubiquitin promoter was used in both constructs to drive gene expression. Transgenic turfgrass plants containing different DNA constructsweregeneratedbyAgrobacterium-mediatedtransformationandanalyzedfortransgeneinsertionandexpression. In replicated in vitro and in vivo experiments under controlled environments, transgenic plants exhibited significantly enhancedresistancetodollarspotandbrownpatch,thetwomajorfungaldiseasesinturfgrass.ThetargetingofPen4-1to endoplasmicreticulumbythetransitpeptideofAP24proteindi