Expression of Genes Encoding Multi-Transmembrane Proteins in Specific Primate Taste Cell Populations 英文参考文献.docVIP

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Expression of Genes Encoding Multi-Transmembrane Proteins in Specific Primate Taste Cell Populations 英文参考文献.doc

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Expression of Genes Encoding Multi-Transmembrane Proteins in Specific Primate Taste Cell Populations 英文参考文献

ExpressionofGenesEncodingMulti-Transmembrane ProteinsinSpecificPrimateTasteCellPopulations BryanD.Moyer.*,PeterHevezi.¤a,NaGao,MinLu,DaliaKalabat,HortensiaSoto¤b,FernandoEcheverri, BiancaLaita,ShaoyangAnthonyYeh,MarkZoller,AlbertZlotnik¤a Senomyx,Inc,SanDiego,California,UnitedStatesofAmerica Abstract Background:Usingfungiform(FG)andcircumvallate(CV)tastebudsisolatedbylasercapturemicrodissectionandanalyzed usinggenearrays,wepreviouslyconstructedacomprehensivedatabaseofgeneexpressioninprimates,whichrevealed over 2,300 taste bud-associated genes. Bioinformatics analyses identified hundreds of genes predicted to encode multi- transmembranedomainproteinswithnopreviousassociationwithtastefunction.Afirststepinelucidatingtherolesthese geneproductsplayingustationistoidentifythespecifictastecelltypesinwhichtheyareexpressed. Methodology/Principal Findings: Using double label in situ hybridization analyses, we identified seven new genes expressedinspecifictastecelltypes,includingsweet,bitter,andumamicells(TRPM5-positive),sourcells(PKD2L1-positive), as well as other taste cell populations. Transmembrane protein 44 (TMEM44), a protein with seven predicted transmembranedomainswithnohomologytoGPCRs,isexpressedinaTRPM5-negativeandPKD2L1-negativepopulation that is enriched in the bottom portion of taste buds and may represent developmentally immature taste cells. Calcium homeostasismodulator1(CALHM1),acomponentofanovelcalciumchannel,alongwithfamilymembersCALHM2and CALHM3; multiple C2 domains; transmembrane 1 (MCTP1), a calcium-binding transmembrane protein; and anoctamin 7 (ANO7),amemberoftherecentlyidentifiedcalcium-gatedchloridechannelfamily,areallexpressedinTRPM5cells.These proteinsmaymodulateandeffectcalciumsignallingstemmingfromsweet,bitter,andumamireceptoractivation.Synaptic vesicleglycoprotein2B(SV2B),aregulatorofsynapticvesicleexocytosis,isexpressedinPKD2L1cells,suggestingthatthis tastecellpopulationtransmitstastantinformationtogustatoryafferentnervefibersviaexocyticneuro