Expression of Signal Transduction System Encoding Genes of Yersinia pseudotuberculosis IP32953 at 28°C and 3°C 英文参考文献.docVIP

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Expression of Signal Transduction System Encoding Genes of Yersinia pseudotuberculosis IP32953 at 28°C and 3°C 英文参考文献.doc

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Expression of Signal Transduction System Encoding Genes of Yersinia pseudotuberculosis IP32953 at 28°C and 3°C 英文参考文献

ExpressionofSignalTransductionSystemEncoding GenesofYersiniapseudotuberculosisIP32953at28 C 6 6 and3 C EveliinaPalonen*,MiiaLindstro¨m,ReijaKarttunen,PanuSomervuo,HannuKorkeala DepartmentofFoodHygieneandEnvironmentalHealth,FacultyofVeterinaryMedicine,UniversityofHelsinki,Helsinki,Finland Abstract Yersinia pseudotuberculosis is a significant psychrotrophic food pathogen whose cold tolerance mechanisms are poorly understood.Signaltransductionsystemsservetomonitortheenvironment,butnosystematicinvestigationoftheirroleat coldtemperaturesinY.pseudotuberculosishasyetbeenundertaken.Therelativeexpressionlevelsof54genespredictedto encodeproteinsbelongingtosignaltransductionsystemsinY.pseudotuberculosisIP32953weredeterminedat28u Cand 3uC by quantitative real-time reverse transcription-PCR. The relative expression levels of 44 genes were significantly (p,0.05) higher at 3uC than at 28uC. Genes encoding the two-component system CheA/CheY had the highest relative expressionlevels at3uC.Mutational analysisrevealed thatcheA is important for growthandmotility at3uC.The relative expressionlevelofonegene,rssB,encodinganRpoSregulator,wassignificantly(p,0.05)lowerat3uCthanat28uC.The results suggest that several signal transduction systems might be used during growth at low temperature, and at least, CheA/CheYtwo-componentsystemisimportantforlow-temperaturegrowth. Citation: Palonen E, Lindstro¨m M, Karttunen R, Somervuo P, Korkeala H (2011) Expression of Signal Transduction System Encoding Genes of Yersinia pseudotuberculosisIP32953at28uCand3uC.PLoSONE6(9):e25063.doi:10.1371/journal.pone.0025063 Editor:UlrichDobrindt,Universita¨t Mu¨nster,Germany ReceivedMarch30,2011;AcceptedAugust26,2011;PublishedSeptember20,2011 Copyright: ? 2011 Palonen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:Thiswor