Expression of Small RNA in Aphis gossypii and Its Potential Role in the Resistance Interaction with Melon 英文参考文献.docVIP

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Expression of Small RNA in Aphis gossypii and Its Potential Role in the Resistance Interaction with Melon 英文参考文献.doc

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Expression of Small RNA in Aphis gossypii and Its Potential Role in the Resistance Interaction with Melon 英文参考文献

ExpressionofSmallRNAinAphisgossypiiandIts PotentialRoleintheResistanceInteractionwithMelon SampurnaSattar1*,CharlesAddo-Quaye2.,YanSong3,4.,JamesA.Anstead1,RamanjuluSunkar3, GaryA.Thompson1* 1College of Agricultural Sciences, The Pennsylvania State University, University Park, Pennsylvania, United States of America, 2The Schatz Center for Tree Molecular Genetics,DepartmentofEcosystemScienceandManagement,ThePennsylvaniaStateUniversity,UniversityPark,Pennsylvania,UnitedStatesofAmerica,3Bioinformatics CoreFacility,OklahomaStateUniversity,Stillwater,Oklahoma,UnitedStatesofAmerica,4DepartmentofBiochemistryandMolecularBiology,OklahomaStateUniversity, Stillwater,Oklahoma,UnitedStatesofAmerica Abstract Background: Theregulatory roleof smallRNAs(sRNAs) invarious biological processes isanactive areaof investigation; however, there has been limited information available on the role of sRNAs in plant-insect interactions. This study was designed to identify sRNAs in cotton-melon aphid (Aphis gossypii) during the Vat-mediated resistance interaction with melon(Cucumismelo). Methodology/Principal Findings: The role of miRNAs was investigated in response to aphid herbivory, during both + resistantandsusceptibleinteractions.sRNAlibrariesmadefromA.gossypiitissuesfeedingonVat andVat2plantsrevealed an unexpected abundance of 27nt long sRNA sequences in the aphids feeding on Vat+ plants. Eighty-one conserved microRNAs(miRNAs),twelveaphid-specificmiRNAs,andninenovelcandidatemiRNAswerealsoidentified.PlantmiRNAs foundintheaphidlibrariesweremostlikelyingestedduringphloemfeeding.ThepresenceofnovelmiRNAswasverifiedby + qPCRexperimentsinbothresistantVat andsusceptibleVat2interactions.Thecomparativeanalysesrevealedthatnovel miRNAs were differentially regulated during the resistant and susceptible interactions. Gene targets predicted for the miRNAsidentifiedinthisstudybyinsilicoanalysesrevealedtheirinvolvementinmorphogenesisandanatomicalstructure determination,signaltransductionpathwa