Filter Paper Blood Spot Enzyme Linked Immunoassay for Insulin and Application in the Evaluation of Determinants of Child Insulin Resistance 英文参考文献.docVIP

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Filter Paper Blood Spot Enzyme Linked Immunoassay for Insulin and Application in the Evaluation of Determinants of Child Insulin Resistance 英文参考文献.doc

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Filter Paper Blood Spot Enzyme Linked Immunoassay for Insulin and Application in the Evaluation of Determinants of Child Insulin Resistance 英文参考文献

FilterPaperBloodSpotEnzymeLinkedImmunoassayfor InsulinandApplicationintheEvaluationof DeterminantsofChildInsulinResistance RichardM.Martin1,2*,RitaPatel1,AlexanderZinovik3,MichaelS.Kramer4,5,EmilyOken6, KonstantinVilchuck3,NataliaBogdanovich3,NataliaSergeichick3,RobertGunnarsson7,LisaGrufman7, YingFoo1,NinaGusina3 1SchoolofSocialandCommunityMedicine,UniversityofBristol,Bristol,UnitedKingdom, 2MRCCentreforCausalAnalysesinTranslationalEpidemiology, Schoolof SocialandCommunityMedicine,UniversityofBristol,Bristol,UnitedKingdom,3NationalResearchandAppliedMedicine,MotherandChildCentre,Minsk,Republicof Belarus,4DepartmentofEpidemiology,BiostatisticsandOccupationalHealth,McGillUniversity,Montreal,Canada,5DepartmentofPediatrics,McGillUniversity,Montreal, Canada, 6Department of Population Medicine, Harvard Medical School and Harvard Pilgrim Health Care Institute, Boston, Massachusetts, United States of America, 7MercodiaAB,Uppsala,Sweden Abstract Background: In large-scale epidemiology, bloodspot sampling by fingerstick onto filter paper has many advantages, includingeaseandlowcostsofcollection,processingandtransport.Wedescribethedevelopmentofanenzyme-linked immunoassay(ELISA)forquantifyinginsulinfromdriedbloodspotsanddemonstrateitsapplicationinalargetrial. Methods:Weadaptedanexistingcommercialkit(MercodiaHumanInsulinELISA,10-1113-01)toquantifyinsulinfromtwo3- mmdiameterdiscs(6mLofblood)punchedfromwholebloodstandardsandfromtrialsamples.Paediatricianscollected driedbloodspotsinafollow-upof13,879fastedchildrenaged11.5years(interquartilerange11.3–11.8years)from31trial sites across Belarus. We quantified bloodspot insulin levels and examined their distribution by demography and anthropometry. Results: Meanintra-assay(n=157)coefficientsofvariationwere15%and6%for ‘low’(6.7mU/L) and‘high’ (23.1mU/L) values, respectively; the respective inter-assay values (n=33) were 23% and 11%. The intraclass correlation coefficient between 50 paired whole bloodspot versus serum