Functional Conservation of Cis-Regulatory Elements of Heat-Shock Genes over Long Evolutionary Distances 英文参考文献.docVIP

FunctionalConservationofCis-RegulatoryElementsof Heat-ShockGenesoverLongEvolutionaryDistances ZhengyingHe,KelsieEichel,IlyaRuvinsky* DepartmentofEcologyandEvolution,InstituteforGenomicsandSystemsBiology,TheUniversityofChicago,Chicago,Illinois,UnitedStatesofAmerica Abstract Transcriptional control of gene regulation is an intricate process that requires precise orchestration of a number of molecular components. Studying its evolution can serve as a useful model for understanding how complex molecular machinesevolve.Onewaytoinvestigateevolutionoftranscriptionalregulationistotestthefunctionsofcis-elementsfrom onespeciesinadistantrelative.Previousresultssuggestedthatfew,ifany,tissue-specificpromotersfromDrosophilaare faithfully expressed in C. elegans. Here we show that, in contrast, promoters of fly and human heat-shock genes are upregulated in C. elegans upon exposure to heat. Inducibility under conditions of heat shock may represent a relatively simple‘‘on-off’’response,whereascomplexexpressionpatternsrequireintegrationofmultiplesignals.Ourresultssuggest thatsimpleraspectsofregulatorylogicmayberetainedoverlongerperiodsofevolutionarytime,whilemorecomplexones maybedivergingmorerapidly. Citation: He Z, Eichel K, Ruvinsky I (2011) Functional Conservation of Cis-Regulatory Elements of Heat-Shock Genes over Long Evolutionary Distances. PLoS ONE6(7):e22677.doi:10.1371/journal.pone.0022677 Editor:JohannesJaeger,CentreforGenomicRegulation(CRG),UniversitatPompeuFabra,Spain ReceivedFebruary23,2011;AcceptedJune30,2011;PublishedJuly25,2011 Copyright:?2011Heetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:Thisworkwasmadepossiblebygrantsupport,awardedtoDr.Ruvinsky,fromtheNationalScienceFoundation(NSF)(IOS-0843504)andtheNational Institutes of Health (NIH) (P50 GM081892). The funders had no role in study design, dat