Functional Screen of Paracrine Signals in Breast Carcinoma Fibroblasts 英文参考文献.docVIP

下载本文档

3
0
约5万字
约 12页
2017-05-11 发布于上海
举报
版权申诉

Functional Screen of Paracrine Signals in Breast Carcinoma Fibroblasts 英文参考文献.doc

1、本文档共12页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Functional Screen of Paracrine Signals in Breast Carcinoma Fibroblasts 英文参考文献

FunctionalScreenofParacrineSignalsinBreast CarcinomaFibroblasts GuiSu1,KyungE.Sung2,DavidJ.Beebe2,AndreasFriedl1,3* 1Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, 2Department of Biomedical Engineering,UniversityofWisconsin-Madison,Madison,Wisconsin,UnitedStatesofAmerica,3PathologyandLaboratoryMedicineService,WilliamS.MiddletonMemorial VeteransHospital,DepartmentofVeteransAffairsMedicalCenter,Madison,Wisconsin,UnitedStatesofAmerica Abstract Stromal fibroblasts actively participate in normal mammary gland homeostasis and in breast carcinoma growth and progressionbysecretingparacrinefactors;however,littleisknownabouttheidentityofparacrinemediatorsinindividual patients.Thepurposeofthisstudywastocharacterizeparacrinesignalingpathwaysbetweenbreastcarcinomacellsand breastcarcinoma-associatedfibroblasts(CAF)ornormalmammaryfibroblasts(NF),respectively.CAFandNFwereisolated from breast carcinoma tissue samples and adjacent normal mammary gland tissue of 28 patients. The fibroblasts were grownin3Dcollagengelco-culturewithT47DhumanbreastcarcinomacellsandT47Dcellgrowthwasmeasured.CAF stimulated T47D cell growth to a significantly greater degree than NF. We detected a considerable inter-individual heterogeneity of paracrine interactions but identified FGF2, HB-EGF, heparanase-1 and SDF1 as factors that were consistently responsible for the activity of carcinoma-associated fibroblasts. CAF from low-grade but not high-grade carcinomas required insulin-like growth factor 1 and transforming growth factor beta 1 to stimulate carcinoma growth. Paradoxically,blockingofmembrane-type1matrixmetalloproteasestimulatedT47Dcellgrowthinco-culturewithNF.The results were largely mirrored by treating the fibroblasts with siRNA oligonucleotides prior to co-culture, implicating the fibroblastsasprincipalproductionsiteforthesecretedmediators.Insummary,weidentifyaparacrinesignalingnetwork withinter-individualcommonaliti