Gene Expression Profiles Identify Inflammatory Signatures in Dendritic Cells 英文参考文献.docVIP

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Gene Expression Profiles Identify Inflammatory Signatures in Dendritic Cells 英文参考文献.doc

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Gene Expression Profiles Identify Inflammatory Signatures in Dendritic Cells 英文参考文献

GeneExpressionProfilesIdentifyInflammatory SignaturesinDendriticCells AnnaTorri1.,OttavioBeretta2.,AnnaRanghetti1,FrancescaGranucci1,PaolaRicciardi-Castagnoli2, MariaFoti1* 1DepartmentofBiotechnologyandBioscience,UniversityofMilano-Bicocca,Milan,Italy,2SingaporeImmunologyNetwork,Singapore,Singapore Abstract Dendritic cells (DCs)constitute aheterogeneousgroupof antigen-presenting leukocytesimportantin activation ofboth innate and adaptive immunity. We studied the gene expression patterns of DCs incubated with reagents inducing their activationorinhibition.TotalRNAwasisolatedfromDCsandgeneexpressionprofilingwasperformedwitholigonucleotide microarrays. Using a supervised learning algorithm based on Random Forest, we generated a molecular signature of inflammationfromatrainingsetof77samples.Wethenvalidatedthismolecularsignatureinatestingsetof38samples. Supervised analysis identified a set of 44 genes that distinguished very accurately between inflammatory and non inflammatory samples. The diagnostic performance of the signature genes was assessed against an independent set of samples,byqRT-PCR.OurfindingssuggestthatthegeneexpressionsignatureofDCscanprovideamolecularclassification foruseintheselectionofanti-inflammatoryoradjuvantmoleculeswithspecificeffectsonDCactivity. Citation:TorriA,BerettaO,RanghettiA,GranucciF,Ricciardi-CastagnoliP,etal.(2010)GeneExpressionProfilesIdentifyInflammatorySignaturesinDendritic Cells.PLoSONE5(2):e9404.doi:10.1371/journal.pone.0009404 Editor:PatriciaT.Bozza,Fundac?a?o OswaldoCruz,Brazil ReceivedSeptember25,2009;AcceptedFebruary4,2010;PublishedFebruary24,2010 Copyright:?2010Torrietal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by the grants from European Commission 6th Framework Program (AIDS-CoVAC), the Italian Ministry of Education and Researc