Genetic Architecture of Aluminum Tolerance in Rice (Oryza sativa) Determined through Genome-Wide Association Analysis and QTL Mapping 英文参考文献.docVIP

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Genetic Architecture of Aluminum Tolerance in Rice (Oryza sativa) Determined through Genome-Wide Association Analysis and QTL Mapping 英文参考文献.doc

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Genetic Architecture of Aluminum Tolerance in Rice (Oryza sativa) Determined through Genome-Wide Association Analysis and QTL Mapping 英文参考文献

GeneticArchitectureofAluminumToleranceinRice (Oryzasativa)DeterminedthroughGenome-Wide AssociationAnalysisandQTLMapping AdamN.Famoso1¤a,KeyanZhao2¤b,RandyT.Clark3,Chih-WeiTung1,MarkH.Wright1,2 ,Carlos Bustamante2¤c,LeonV.Kochian3,SusanR.McCouch1* 1DepartmentofPlantBreedingandGenetics,CornellUniversity,Ithaca,NewYork,UnitedStatesofAmerica,2DepartmentofBiologicalStatisticsandComputational Biology,CornellUniversity,Ithaca,NewYork,UnitedStatesofAmerica,3RobertW.HolleyCenterforAgricultureandHealth,AgriculturalResearchService,USDepartment ofAgriculture,CornellUniversity,Ithaca,NewYork,UnitedStatesofAmerica Abstract Aluminum (Al) toxicity is a primary limitation to crop productivity on acid soils, and rice has been demonstrated to be significantlymoreAltolerantthanothercerealcrops.However,themechanismsofriceAltolerancearelargelyunknown, and no genes underlying natural variation have been reported. We screened 383 diverse rice accessions, conducted a genome-wideassociation(GWA)study,andconductedQTLmappingintwobi-parentalpopulationsusingthreeestimates ofAltolerancebasedonrootgrowth.Subpopulationstructureexplained57%ofthephenotypicvariation,andthemeanAl tolerance in Japonica was twice that of Indica. Forty-eight regions associated with Al tolerance were identified by GWA analysis,mostofwhichweresubpopulation-specific.Fouroftheseregionsco-localizedwithaprioricandidategenes,and two highly significant regions co-localized with previously identified QTLs. Three regions corresponding to induced Al- sensitive rice mutants (ART1, STAR2, Nrat1) were identified through bi-parental QTL mapping or GWA to be involved in naturalvariationforAltolerance.HaplotypeanalysisaroundtheNrat1geneidentifiedsusceptibleandtoleranthaplotypes explaining40%oftheAltolerancevariationwithintheaussubpopulation,andsequenceanalysisofNrat1identifiedatrioof non-synonymousmutationspredictiveofAlsensitivityinourdiversitypanel.GWAanalysisdiscoveredmorephenotype– genotype associations and provided higher resolutio