Gene Expression Signatures of Extracellular Matrix and Growth Factors during Embryonic Stem Cell Differentiation 英文参考文献.docVIP

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Gene Expression Signatures of Extracellular Matrix and Growth Factors during Embryonic Stem Cell Differentiation 英文参考文献.doc

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Gene Expression Signatures of Extracellular Matrix and Growth Factors during Embryonic Stem Cell Differentiation 英文参考文献

GeneExpressionSignaturesofExtracellularMatrixand GrowthFactorsduringEmbryonicStemCell Differentiation RekhaNair1.,AlyssaV.Ngangan1.,MelissaL.Kemp1,2,ToddC.McDevitt1,2 * 1TheWallaceH.CoulterDepartmentofBiomedicalEngineering,GeorgiaInstituteofTechnologyandEmoryUniversity,Atlanta,Georgia,UnitedStatesofAmerica,2The ParkerH.PetitInstituteforBioengineeringandBioscience,GeorgiaInstituteofTechnology,Atlanta,Georgia,UnitedStatesofAmerica Abstract Pluripotent stem cells are uniquely capable of differentiating into somatic cell derivatives of all three germ lineages, thereforeholdingtremendouspromisefordevelopmentalbiologystudiesandregenerativemedicinetherapies.Although temporal patterns of phenotypic gene expression have been relatively well characterized during the course of differentiation,coincidentpatternsofendogenousextracellularmatrix(ECM)andgrowthfactorexpressionthataccompany pluripotentstemcelldifferentiationremainmuchlesswell-defined.Thus,theobjectiveofthisstudywastoexaminethe globaldynamicprofilesofECMandgrowthfactorgenesassociatedwithearlystagesofpluripotentmouseembryonicstem cell(ESC)differentiation.GeneexpressionanalysisofECMandgrowthfactorsbyESCsdifferentiatingasembryoidbodiesfor upto14dayswasassessedusingPCRarrays(172uniquegenestotal),andtheresultswereexaminedusingavarietyofdata mining methods. As expected, decreases in the expression of genes regulating pluripotent stem cell fate preceded subsequentincreasesinmorphogenexpressionassociatedwithdifferentiation.PathwayanalysisgeneratedsolelyfromECM and growth factor gene expression highlighted morphogenic cell processes within the embryoid bodies, such as cell growth, migration, and intercellular signaling, that are required for primitive tissue and organ developmental events. In addition,systemsanalysisofECMandgrowthfactorgeneexpressionaloneidentifiedintracellularmoleculesandsignaling pathwaysinvolvedintheprogressionofpluripotentstemcelldifferentiationthatwerenotcontainedwithinthearraydata set. Overall, these