Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene 英文参考文献.docVIP

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Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene 英文参考文献.doc

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Genetic Basis of Growth Adaptation of Escherichia coli after Deletion of pgi, a Major Metabolic Gene 英文参考文献

GeneticBasisofGrowthAdaptationofEscherichiacoli afterDeletionofpgi,aMajorMetabolicGene PepCharusanti1,TomM.Conrad1,EricM.Knight1¤,KarthikVenkataraman1,NicoleL.Fong1,BinXie2, YuanGao2,3,Bernhard?.Palsson1* 1DepartmentofBioengineering,UniversityofCaliforniaSanDiego,LaJolla,California,UnitedStatesofAmerica,2CenterfortheStudyofBiologicalComplexity,Virginia Commonwealth University, Richmond, Virginia, United States of America, 3Departmentof Computer Science, VirginiaCommonwealth University,Richmond, Virginia, UnitedStatesofAmerica Abstract Bacterialsurvivalrequiresadaptationtodifferentenvironmentalperturbationssuchasexposuretoantibiotics,changesin temperature or oxygen levels, DNAdamage, andalternative nutrient sources. During adaptation, bacteria often develop beneficialmutationsthatconferincreasedfitnessinthenewenvironment.Adaptationtothelossofamajornon-essential geneproductthatcripplesgrowth,however,hasnotbeenstudiedatthewhole-genomelevel.Weinvestigatedtheability of Escherichia coli K-12 MG1655 to overcome the loss of phosphoglucose isomerase (pgi) by adaptively evolving ten replicatesofE.colilackingpgifor50daysinglucoseM9minimalmediumandbycharacterizingendpointclonesthrough whole-genome re-sequencing and phenotype profiling. We found that 1) the growth rates for all ten endpoint clones increasedapproximately3-foldoverthe50-dayperiod;2)twotofivemutationsaroseduringadaptation,mostfrequentlyin theNADH/NADPHtranshydrogenasesudhAandpntABandinthestress-associatedsigmafactorrpoS;and3)despitesimilar growthrates,atleastthreedistinctendpointphenotypesdevelopedasdefinedbydifferentratesofacetateandformate secretion.TheseresultsdemonstratethatE.colicanadapttothelossofamajormetabolicgeneproductwithonlyahandful ofmutationsandthatadaptationcanresultinmultiple,alternativephenotypes. Citation:CharusantiP,ConradTM,KnightEM,VenkataramanK,FongNL,etal.(2010)GeneticBasisofGrowthAdaptationofEscherichiacoliafterDeletionofpgi, aMajorMetabolicGene.PLoSGenet6(11):e1001186.doi:10.1371/journal.