Genome-Wide Identification of Reverse Complementary microRNA Genes in Plants 英文参考文献.docVIP

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Genome-Wide Identification of Reverse Complementary microRNA Genes in Plants 英文参考文献.doc

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Genome-Wide Identification of Reverse Complementary microRNA Genes in Plants 英文参考文献

Genome-WideIdentificationofReverseComplementary microRNAGenesinPlants ChaogangShao1.,XiaoxiaMa2.,XiufangXu1,HuizhongWang2*,YijunMeng2* 1CollegeofLifeSciences,HuzhouTeachersCollege,Huzhou,ThePeople’sRepublicofChina,2CollegeofLifeandEnvironmentalSciences,HangzhouNormalUniversity, Hangzhou,ThePeople’sRepublicofChina Abstract MicroRNAs(miRNAs)are,21-nucleotidesmallRNAs(sRNAs)withessentialregulatoryrolesinplants.Theyaregenerated fromstem-loop-structuredprecursorsthroughtwosequentialDicer-like1(DCL1)-mediatedcleavages.Todate,hundredsof plant miRNAs havebeen uncovered. However, the question, whether thesequences reverse complementary (RC) to the miRNAprecursorscouldformhairpin-likestructuresandproducesRNAduplexessimilartothemiRNA/miRNA*pairshasnot beensolvedyet.Here,weinterrogatedthispossibilityin16plantspeciesbasedonsRNAhigh-throughputsequencingdata and secondary structure prediction. A total of 59RC sequences with great potential to form stem-loop structures and generatemiRNA/miRNA*-likeduplexeswereidentifiedintenplants,whichwerenamedasRC-miRNAprecursors.Unlikethe canonicalmiRNAs,onlyafewcleavagetargetsoftheRC-miRNAswereidentifiedinArabidopsis(Arabidopsisthaliana )and rice (Oryza sativa), and none in Soybean (Glycine max) based on degradome data. Surprisingly, the genomic regions surroundingsomeoftheRC-miRNAtargetrecognitionsiteswereobservedtobespecificallymethylatedinbothArabidopsis andrice.Takentogether,wereportedanewclassofmiRNAs,calledRC-miRNAs,whichweregeneratedfromtheantisense strands of the miRNA precursors. Based on the results, we speculated that the mature RC-miRNAs might have subtle regulatory activity through target cleavages, but might possess short interfering RNA-like activity by guiding sequence- specificDNAmethylation. Citation:ShaoC,MaX,XuX,WangH,MengY(2012)Genome-WideIdentificationofReverseComplementarymicroRNAGenesinPlants.PLoSONE7(10):e46991. doi:10.1371/journal.pone.0046991 Editor:BaohongZhang,EastCarolinaUniversity,UnitedStatesofAmerica ReceivedA