Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica 英文参考文献.docVIP

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Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica 英文参考文献.doc

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Genome Wide Expression Profiling Reveals Suppression of Host Defence Responses during Colonisation by Neisseria meningitides but not N. lactamica 英文参考文献

GenomeWideExpressionProfilingRevealsSuppression ofHostDefenceResponsesduringColonisationby NeisseriameningitidesbutnotN.lactamica HazelEnEnWong1,2,Ming-ShiLi2,J.SimonKroll2,MartinL.Hibberd1,PaulR.Langford2* 1InfectiousDiseases,GenomeInstituteofSingapore,Singapore,Singapore,2SectionofPaediatrics,ImperialCollegeLondon,London,UnitedKingdom Abstract BothNeisseriameningitidisandthecloselyrelatedbacteriumNeisserialactamicacolonisehumannasopharyngealmucosal surface,butonlyN.meningitidisinvadesthebloodstreamtocausepotentiallylife-threateningmeningitisandsepticaemia. Wehavehypothesisedthatthetwoneisserialspeciesdifferentiallymodulatehostrespiratoryepithelialcellgeneexpression reflectingtheirdiseasepotential.Confluentmonolayersof16HBE14humanbronchialepithelialcellswereexposedtolive and/ordeadN.meningitidis(includingcapsuleandpilimutants)andN.lactamica,andtheirtranscriptomeswerecompared usingwholegenomemicroarrays.ChangesinexpressionofselectedgenesweresubsequentlyvalidatedusingQ-RT-PCR andELISAs.LiveN.meningitidisandN.lactamicainducedgenesinvolvedinhostenergyproductionprocessessuggesting that both bacterial species utilise host resources. N. meningitidis infection was associated with down-regulation of host defencegenes.N.lactamica,relativetoN.meningitidis,initiatesup-regulationofproinflammatorygenes.Bacterialsecreted proteinsaloneinducedsomeofthechangesobserved.TheresultssuggestN.meningitidisandN.lactamicadifferentially regulatehostrespiratoryepithelialcellgeneexpressionthroughcolonisationand/orproteinsecretion,andthatthismay contributetosubsequentclinicaloutcomesassociatedwiththesebacteria. Citation:WongHEE,LiM-S,KrollJS,HibberdML,LangfordPR(2011)GenomeWideExpressionProfilingRevealsSuppressionofHostDefenceResponsesduring ColonisationbyNeisseriameningitidesbutnotN.lactamica.PLoSONE6(10):e26130.doi:10.1371/journal.pone.0026130 Editor:RayBorrow,HealthProtectionAgency,UnitedKingdom ReceivedJune2,2011;AcceptedSeptember20,2011;PublishedOctober20,2011 Copyright: ? 2011 Wong et al