Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142 英文参考文献.docVIP

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Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142 英文参考文献.doc

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Genome-Scale Modeling of Light-Driven Reductant Partitioning and Carbon Fluxes in Diazotrophic Unicellular Cyanobacterium Cyanothece sp. ATCC 51142 英文参考文献

Genome-ScaleModelingofLight-DrivenReductant PartitioningandCarbonFluxesinDiazotrophic UnicellularCyanobacteriumCyanothecesp.ATCC51142 TrangT.Vu1.,SergeyM.Stolyar2.,GrigoriyE.Pinchuk2,EricA.Hill2,LeoA.Kucek2,RoslynN.Brown2, MaryS.Lipton2,AndreiOsterman3,JimK.Fredrickson2,AllanE.Konopka2,AlexanderS.Beliaev2*, JenniferL.Reed1* 1Department ofChemicalandBiologicalEngineering,University ofWisconsin- Madison,Madison,Wisconsin,UnitedStates ofAmerica, 2Biological SciencesDivision, Pacific NorthwestNationalLaboratory,Richland, Washington,UnitedStatesofAmerica, 3Burnham InstituteforMedicalResearch, LaJolla,California,UnitedStatesof America Abstract Genome-scalemetabolicmodelshaveprovenusefulforansweringfundamentalquestionsaboutmetaboliccapabilitiesofa varietyofmicroorganisms,aswellasinformingtheirmetabolicengineering.However,onlyafewmodelsareavailablefor oxygenic photosynthetic microorganisms, particularly in cyanobacteria in which photosynthetic and respiratory electron transportchains(ETC)sharecomponents.WeaddressedthecomplexityofcyanobacterialETCbydevelopingagenome- scale model for the diazotrophic cyanobacterium, Cyanothece sp. ATCC 51142. The resulting metabolic reconstruction, iCce806,consistsof806genesassociatedwith667metabolicreactionsandincludesadetailedrepresentationoftheETC and a biomass equation based on experimental measurements. Both computational and experimental approaches were usedtoinvestigatelight-drivenmetabolisminCyanothecesp.ATCC51142,withaparticularfocusonreductantproduction and partitioning within the ETC. The simulation results suggest that growth and metabolic flux distributions are substantiallyimpactedbytherelativeamountsoflightgoingintotheindividualphotosystems.Whengrowthislimitedby thefluxthroughphotosystemI,terminalrespiratoryoxidasesarepredictedtobeanimportantmechanismforremoving excessreductant.Similarly,underphotosystemIIfluxlimitation,excesselectroncarriersmustberemovedviacyclicelectron transport.Furthermore,insilicocalculationswer