Genome-Wide Identification of Small RNAs in the Opportunistic Pathogen Enterococcus faecalis V583 英文参考文献.docVIP

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Genome-Wide Identification of Small RNAs in the Opportunistic Pathogen Enterococcus faecalis V583 英文参考文献.doc

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Genome-Wide Identification of Small RNAs in the Opportunistic Pathogen Enterococcus faecalis V583 英文参考文献

Genome-WideIdentificationofSmallRNAsinthe OpportunisticPathogenEnterococcusfaecalisV583 KoukiShioya1,CharlotteMichaux1,CarstenKuenne2,TorstenHain2,NicolasVerneuil1,Aure′lie Budin-Verneuil1,ThomasHartsch3,AxelHartke1,Jean-ChristopheGiard1* 1LaboratoiredeMicrobiologiedel’Environnement,EA956-USCINRA2017-IFR146ICORE,UniversityofCaen,Caen,France, 2InstituteofMedicalMicrobiology, Justus- Liebig-University,Giessen,Germany,3GenedataAG,Basel,Switzerland Abstract SmallRNAmolecules(sRNAs)arekeymediatorsofvirulenceandstressinduciblegeneexpressionsinsomepathogens.In thisworkweidentifysRNAsintheGrampositiveopportunisticpathogenEnterococcusfaecalis.Wecharacterized11sRNAs by tiling microarray analysis, 59 and 39 RACE-PCR, and Northern blot analysis. Six sRNAs were specifically expressed at exponentialphase,twosRNAswereobservedatstationaryphase,andthreeweredetectedduringbothphases.Searchesof putative functions revealed that three of them (EFA0080_EFA0081 and EFB0062_EFB0063 on pTF1 and pTF2 plasmids, respectively,andEF0408_EF04092locatedonthechromosome)aresimilartoantisenseRNAinvolvedinplasmidaddiction modules.Moreover,EF1097_EF1098sharesstronghomologieswithtmRNA(bi-functionalRNAactingasbothatRNAandan mRNA) and EF2205_EF2206 appears homologous to 4.5S RNA member of the Signal Recognition Particle (SRP) ribonucleoproteincomplex.Inaddition,proteomicanalysisoftheDEF3314_EF3315sRNAmutantsuggeststhatitmaybe involvedintheturnoverofsomeabundantproteins.Theexpressionpatternsofthesetranscriptswereevaluatedbytiling array hybridizations performed with samples from cells grown under eleven different conditions some of which may be encounteredduringinfection.Finally,distributionofthesesRNAsamonggenomesequencesof54E.faecalisstrainswas assessed. This is the first experimental genome-wide identification of sRNAs in E. faecalis and provides impetus to the understandingofgeneregulationinthisimportanthumanpathogen. Citation:ShioyaK,MichauxC,KuenneC,HainT,VerneuilN,etal.(2011)Genome-WideIde