Homeobox Transcription Factors Are Required for Conidiation and Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae 英文参考文献.docVIP

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Homeobox Transcription Factors Are Required for Conidiation and Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae 英文参考文献.doc

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Homeobox Transcription Factors Are Required for Conidiation and Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae 英文参考文献

HomeoboxTranscriptionFactorsAreRequiredfor ConidiationandAppressoriumDevelopmentintheRice BlastFungusMagnaportheoryzae SeryunKim,Sook-YoungPark,KyoungSuKim,Hee-SoolRho,Myoung-HwanChi,JaehyukChoi, JongsunPark,SunghyungKong,JaejinPark,JaedukGoh,Yong-HwanLee* DepartmentofAgriculturalBiotechnology,CenterforFungalGeneticResourcesandCenterforFungalPathogenesis,SeoulNationalUniversity,Seoul,Korea Abstract Theappropriatedevelopmentofconidiaandappressoriaiscriticalinthediseasecycleofmanyfungalpathogens,including Magnaportheoryzae.Atotalofeightgenes(MoHOX1toMoHOX8)encodingputativehomeoboxtranscriptionfactors(TFs) were identified from the M. oryzae genome. Knockout mutants for each MoHOX gene were obtained via homology- dependent gene replacement. Two mutants, DMohox3 and DMohox5, exhibited no difference to wild-type in growth, conidiation, conidium size, conidial germination, appressorium formation, and pathogenicity. However, the DMohox1 showed a dramatic reduction in hyphal growth and increase in melanin pigmentation, compared to those in wild-type. DMohox4 and DMohox6 showed significantly reduced conidium size and hyphal growth, respectively. DMohox8 formed normal appressoria, but failed in pathogenicity, probably due to defects in the development of penetration peg and invasivegrowth.ItismostnotablethatasexualreproductionwascompletelyabolishedinDMohox2,inwhichnoconidia formed. DMohox2 was still pathogenic through hypha-driven appressoria in a manner similar to that of the wild-type. However,DMohox7wasunabletoformappressoriaeitheronconidialgermtubes,orathyphaltips,beingnon-pathogenic. These factors indicate that M. oryzae is able to cause foliar disease via hyphal appressorium-mediated penetration, and MoHOX7 is mutually required to drive appressorium formation from hyphae and germ tubes. Transcriptional analyses suggest that the functioning of M. oryzae homeobox TFs is mediated through the regulation of gene expression and is 2+ affectedbycAMPandCa signalingand/orMA