Mutations in Escherichia coli aceE and ribB Genes Allow Survival of Strains Defective in the First Step of the Isoprenoid Biosynthesis Pathway 英文参考文献.docVIP
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Mutations in Escherichia coli aceE and ribB Genes Allow Survival of Strains Defective in the First Step of the Isoprenoid Biosynthesis Pathway 英文参考文献
MutationsinEscherichiacoliaceEandribBGenesAllow
SurvivalofStrainsDefectiveintheFirstStepofthe
IsoprenoidBiosynthesisPathway
JordiPerez-Gil1,EvaMariaUros1,SusannaSauret-Gu¨eto1¤,L.MariaLois1,JamesKirby2,
MinobuNishimoto2,EdwardE.K.Baidoo2,JayD.Keasling2,AlbertBoronat1,3,ManuelRodriguez-
Concepcion1*
1Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, Barcelona, Spain, 2Joint BioEnergy Institute, Emeryville, California,
UnitedStatesofAmerica,3DepartmentdeBioqu?′micaiBiologiaMolecular,UniversitatdeBarcelona,Barcelona,Spain
Abstract
Afunctional2-C-methyl-D-erythritol4-phosphate(MEP)pathwayisrequiredforisoprenoidbiosynthesisandhencesurvival
inEscherichiacoliandmostotherbacteria.Inthefirsttwostepsofthepathway,MEPisproducedfromthecentralmetabolic
intermediatespyruvateandglyceraldehyde3-phosphatevia1-deoxy-D-xylulose5-phosphate(DXP)bytheactivityofthe
enzymesDXPsynthase(DXS)andDXPreductoisomerase(DXR).BecausetheMEPpathwayisabsentfromhumans,itwas
proposedasapromisingnewtargettodevelopnewantibiotics.However,thelethalphenotypecausedbythedeletionof
DXSorDXRwasfoundtobesuppressedwitharelativelyhighefficiencybyunidentifiedmutations.Herewereportthat
several mutations in the unrelated genes aceE and ribB rescue growth of DXS-defective mutants because the encoded
enzymesallowedtheproductionofsufficientDXPinvivo.Together,thisworkunveilsthediversityofmechanismsthatcan
evolveinbacteriatocircumventablockageofthefirststepoftheMEPpathway.
Citation:Perez-GilJ,UrosEM,Sauret-Gu¨etoS,LoisLM,KirbyJ,etal.(2012)MutationsinEscherichiacoliaceEandribBGenesAllowSurvivalofStrainsDefectivein
theFirstStepoftheIsoprenoidBiosynthesisPathway.PLoSONE7(8):e43775.doi:10.1371/journal.pone.0043775
Editor:JohnMcCutcheon,UniversityOfMontana–Missoula,UnitedStatesofAmerica
ReceivedJune22,2012;AcceptedJuly25,2012;PublishedAugust21,2012
Copyright: ? 2012 Perez-Gil et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License,
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