Non-Invasive Detection of a Small Number of Bioluminescent Cancer Cells In Vivo 英文参考文献.docVIP
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Non-Invasive Detection of a Small Number of Bioluminescent Cancer Cells In Vivo 英文参考文献
Non-InvasiveDetectionofaSmallNumberof
BioluminescentCancerCellsInVivo
Jae-BeomKim1*,KonnieUrban1,EdwardCochran3,SteveLee1,AngelAng1,BradleyRice1,AdamBata2,
KennethCampbell2,RichardCoffee2,AlexGorodinsky2,ZhanLu2,HeZhou3,TakashiKeiKishimoto3,
PeterLassota1*
1Caliper Life Sciences Inc., Alameda, California, United States of America, 2Caliper Life Sciences Inc., Cranbury, New Jersey, United States of America, 3Momenta
PharmaceuticalsInc.,Cambridge,Massachusetts,UnitedStatesofAmerica
Abstract
Early detection of tumors can significantly improve the outcome of tumor treatment. One of the most frequently asked
questionsincancerimagingishowmanycellscanbedetectednon-invasivelyinaliveanimal.Althoughmanyfactorslimit
suchdetection,increasingthelightemissionfromcellsisoneofthemosteffectivewaysofovercomingtheselimitations.
Here,wedescribedevelopmentandutilizationofalentiviralvectorcontainingenhancedfireflyluciferase(luc2)gene.The
resultingsinglecellclonesofthemousemammaryglandtumor(4T1-luc2)showedstablelightemissionintherangeof
10,000photons/sec/cell.Insomecasesindividual4T1-luc2cellsinsertedundertheskinofanu/numousecouldbedetected
non-invasivelyusingacooledCCDcamerainsomecases.Inaddition,weshowedthatonlyfewcellsareneededtodevelop
tumors in these mice and tumor progression can be monitored right after the cells are implanted. Significantly higher
luciferaseactivityinthesecellsallowedustodetectmicrometastasesinboth,syngeneicBalb/candnu/numice.
Citation:KimJ-B,UrbanK,CochranE,LeeS,AngA,etal.(2010)Non-InvasiveDetectionofaSmallNumberofBioluminescentCancerCellsInVivo.PLoSONE5(2):
e9364.doi:10.1371/journal.pone.0009364
Editor:AlexanderSwarbrick,GarvanInstituteofMedicalResearch,Australia
ReceivedMay12,2009;AcceptedJanuary2,2010;PublishedFebruary23,2010
Copyright:?2010Kimetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted
use,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited.
Funding:
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