Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 英文参考文献.docVIP

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Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 英文参考文献.doc

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Non-Sterilized Fermentative Production of Polymer-Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 英文参考文献

Non-SterilizedFermentativeProductionofPolymer- Grade L-LacticAcidbyaNewlyIsolatedThermophilic StrainBacillussp.2–6 JiayangQin1,3,BoZhao2,XiuwenWang1,3,LiminWang1,2,BoYu2,YanheMa1,2,CuiqingMa3 ,Hongzhi Tang4,JibinSun1,PingXu1,3,4* 1TianjinIndustrialBiotechnologyRDCenter,ChineseAcademyofSciences,Tianjin,People’sRepublicofChina,2InstituteofMicrobiology,ChineseAcademyofSciences, Beijing,People’sRepublicofChina,3StateKeyLaboratoryofMicrobialTechnology,ShandongUniversity,Jinan,People’sRepublicofChina,4KeyLaboratoryofMicrobial Metabolism,MinistryofEducation,SchoolofLifeSciencesandBiotechnology,ShanghaiJiaoTongUniversity,Shanghai,People’sRepublicofChina Abstract Background:Thedemandforlacticacidhasbeenincreasingconsiderablybecauseofitsuseasamonomerforthesynthesis ofpolylacticacid(PLA),whichisapromisingandenvironment-friendlyalternativetoplasticsderivedfrompetrochemicals. Optically pure L-lactic acid is essential for polymerization of PLA. The high fermentation cost of L-lactic acid is another limitationforPLApolymerstocompetewithconventionalplastics. Methodology/Principal Findings: A Bacillus sp. strain 2–6 for production of L-lactic acid was isolated at 55uC from soil samples. Itsthermophilic characteristic madeitagood lactic acid producer becauseoptically pure L-lactic acid couldbe producedbythisstrainunderopenconditionwithoutsterilization.In5-literbatchfermentationofBacillussp.2–6,118.0g/ literof L-lacticacidwithanopticalpurityof99.4%wasobtainedfrom121.3g/literofglucose.Theyieldwas97.3%andthe averageproductivitywas4.37g/liter/h.Themaximum L-lacticacidconcentrationof182.0g/literwasobtainedfrom30-liter fed-batchfermentationwithanaverageproductivityof3.03g/liter/handproductopticalpurityof99.4%. Conclusions/Significance:WiththenewlyisolatedBacillussp.strain2–6,highconcentrationofopticallypure L-lacticacid couldbeproducedefficientlyinopenfermentationwithoutsterilization,whichwouldleadtoanewcost-effectivemethod forpolymer-grade L-lacticacidproductionfromrenewabl