Nuclear Import and Dimerization of Tomato ASR1, a Water Stress-Inducible Protein Exclusive to Plants 英文参考文献.docVIP
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Nuclear Import and Dimerization of Tomato ASR1, a Water Stress-Inducible Protein Exclusive to Plants 英文参考文献
NuclearImportandDimerizationofTomatoASR1,a
WaterStress-InducibleProteinExclusivetoPlants
MartinianoM.Ricardi1,FranciscoF.Guaimas2,RodrigoM.Gonza′lez1,Herna′nP.Burrieza3,
Mar?′aP.Lo′pez-Ferna′ndez3,ElizabethA.Jares-Erijman2{,Jose′ M.Este′vez1,NorbertoD.Iusem1,4*
1Instituto de Fisiolog?′a, Biolog?′a Molecular y Neurociencias (IFIByNE-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires,
Argentina, 2Departamento de Qu?′mica Orga′nica and CIHIDECAR, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires,
Argentina,3Departamento deBiodiversidadyBiolog?′aExperimental,FacultaddeCienciasExactasyNaturales,UniversidaddeBuenosAires,BuenosAires,Argentina,
4Laboratorio de Fisiolog?′a y Biolog?′a Molecular (LFBM), Departamento de Fisiolog?′a, Biolog?′a Molecular y Celular (FBMC), Facultad de Ciencias Exactas y Naturales,
UniversidaddeBuenosAires,BuenosAires,Argentina
Abstract
TheASR(forABA/waterstress/ripening)proteinfamily,firstdescribedintomatoasnuclearandinvolvedinadaptationto
dryclimates,iswidespreadintheplantkingdom,includingcropsofhighagronomicrelevance.Weshowbothnuclearand
cytosoliclocalizationforASR1(themoststudiedmemberofthefamily)inhistologicalplantsamplesbyimmunodetection,
typicallyfoundinsmallproteinsreadilydiffusingthroughnuclearpores.Indeed,anuclearlocalizationwasexpectedbased
onsortingpredictionsoftware,whichalsohighlightamonopartitenuclearlocalizationsignal(NLS)intheprimarysequence.
However,hereweprovethatsuchan‘‘NLS’’ofASR1fromtomatoisdispensableandnon-functional,beingthetransportof
the protein to thenucleus due tosimple diffusionacross nuclear pores. We attribute such atargeting deficiency to the
misplacing in that cryptic NLS of two conserved contiguous lysine residues. Based on previous in vitro experiments
regardingquaternarystructure,wealsocarriedoutlivecellimagingassaysthroughconfocalmicroscopytoexploredimer
formationinplanta.Wefoundhomodimersinboththecytosolandthenucleusa
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