Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems 英文参考文献.docVIP
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Nucleic Acid-based Detection of Bacterial Pathogens Using Integrated Microfluidic Platform Systems 英文参考文献
Sensors 2009, 9, 3713-3744; doi:10.3390/OPEN ACCESS
sensors
ISSN 1424-8220
/journal/sensors
Review
Nucleic Acid-based Detection of Bacterial Pathogens Using
Integrated Microfluidic Platform Systems
Clarissa Lui 1,*, Nathaniel C. Cady 2 and Carl A. Batt 3
1
Department of Biomedical Engineering / Cornell University, 317 Stocking Hall, Ithaca, NY 14853,
USA
2
College of Nanoscale Science and Engineering / University at Albany State University of New
York, 255 Fuller Rd., Albany, NY 12203, USA; E-Mail: ncady@ (N.C.C.)
3
Department of Food Science / Cornell University, 312 Stocking Hall, Ithaca, NY 14853, USA;
E-Mail: cab10@ (C.A.B.)
* Author to whom correspondence should be addressed; E-Mail: csl42@;
Tel. +1-607-255-7902; Fax: +1-607-255-8741
Received: 9 April 2009; in revised form: 12 May 2009 / Accepted: 18 May 2009 /
Published: 18 May 2009
Abstract: The advent of nucleic acid-based pathogen detection methods offers increased
sensitivity and specificity over traditional microbiological techniques, driving the
development of portable, integrated biosensors. The miniaturization and automation of
integrated detection systems presents a significant advantage for rapid, portable field-based
testing. In this review, we highlight current developments and directions in nucleic acid-
based micro total analysis systems for the detection of bacterial pathogens. Recent progress
in the miniaturization of microfluidic processing steps for cell capture, DNA extraction and
purification, polymerase chain reaction, and product detection are detailed. Discussions
include strategies and challenges for implementation of an integrated portable platform.
Keywords: pathogen; sensors; biosensors; PCR; DNA; microfluidics; μTAS; lab-on-a-
chip
Sensors 2009, 9
3714
1. Introduction
The rapid, reliable detection of pathogenic bacteria is imperative in many different industries, of
which food and agriculture, healthcare, env
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