Optical Biomarkers of Serous and Mucinous Human Ovarian Tumor Assessed with Nonlinear Optics Microscopies 英文参考文献.docVIP

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Optical Biomarkers of Serous and Mucinous Human Ovarian Tumor Assessed with Nonlinear Optics Microscopies 英文参考文献.doc

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Optical Biomarkers of Serous and Mucinous Human Ovarian Tumor Assessed with Nonlinear Optics Microscopies 英文参考文献

OpticalBiomarkersofSerousandMucinousHuman OvarianTumorAssessedwithNonlinearOptics Microscopies JavierAdur1,2*,VitorB.Pelegati1,AndreA.deThomaz1,MarianaO.Baratti6,DiogoB.Almeida1, L.A.L.A.Andrade3,Fa′timaBottcher-Luiz4,HernandesF.Carvalho5,6,CarlosL.Cesar1,6 1BiophotonicGroup,OpticsandPhotonicsResearchCenter(CEPOF),InstituteofPhysics‘‘GlebWataghin’’,StateUniversityofCampinas–UNICAMP,Campinas,Sa?oPaulo, Brazil,2MicroscopyLaboratoryAppliedtoMolecularandCellularStudies,SchoolofBioengineering,NationalUniversityofEntreR?′os,OroVerde,EntreR?′os,Argentina, 3DepartmentofPathologicalAnatomy,FacultyofMedicalSciences,StateUniversityofCampinas–UNICAMP,Campinas,Sa?o Paulo,Brazil,4DepartmentofObstetrics andGynecology,FacultyofMedicalSciences,StateUniversityofCampinas–UNICAMP,Campinas,Sa?o Paulo,Brazil,5DepartmentofAnatomy,CellBiology,Physiology andBiophysics,InstituteofBiology,StateUniversityofCampinas–UNICAMP,Campinas,Sa?o Paulo,Brazil,6INFABiC-NationalInstituteofScienceandTechnologyon PhotonicsAppliedtoCellBiology,Campinas,Sa?o Paulo,Brazil Abstract Background: Nonlinear optical (NLO) microscopy techniques have potential to improve the early detection of epithelial ovariancancer.InthisstudyweshowedthatmultimodalNLOmicroscopies,includingtwo-photonexcitationfluorescence (TPEF),second-harmonicgeneration(SHG),third-harmonicgeneration(THG)andfluorescencelifetimeimagingmicroscopy (FLIM)candetectmorphologicalandmetabolicchangesassociatedwithovariancancerprogression. Methodology/Principal Findings: We obtained strong TPEF + SHG + THG signals from fixed samples stained with HematoxylinEosin(HE)androbustFLIMsignalfromfixedunstainedsamples.Particularly,weimaged34ovarianbiopsies fromdifferentpatients(medianage,49years)including5normalovariantissue,18seroustumorsand11mucinoustumors withthemultimodalNLOplatformdevelopedinourlaboratory.Wehavebeenabletodistinguishadenomas,borderline, andadenocarcinomasspecimens.Usingacompletesetofscoringmethodswefoundsignificantdifferen