Optical Sensing Method for Screening Disease in Melon Seeds by Using Optical Coherence Tomography 英文参考文献.docVIP

Sensors 2011, 11, 9467-9477; doi:10.3390/s111009467 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Optical Sensing Method for Screening Disease in Melon Seeds by Using Optical Coherence Tomography Changho Lee 1, Seung-Yeol Lee 2, Jeong-Yeon Kim 3, Hee-Young Jung 2 and Jeehyun Kim 1,* 1 School of Electrical Engineering and Computer Science, Kyungpook National University, 1370, Sankyuk-dong, Buk-gu, Daegu 702-701, Korea; E-Mail: song31037@knu.ac.kr 2 School of Applied Biosciences, Kyungpook National University, 1370, Sankyuk-dong, Buk-gu, Daegu 702-701, Korea; E-Mails: leesy1985@ (S.-Y.L.); heeyoung@knu.ac.kr (H.-Y.J.) 3 Division of General Studies, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea * Author to whom correspondence should be addressed; E-Mail: jeehk@knu.ac.kr; Tel.: +82-53-950-7221; Fax: +82-53-950-7221. Received: 17 August 2011; in revised form: 21 September 2011 / Accepted: 22 September 2011 / Published: 10 October 2011 Abstract: We report a noble optical sensing method to diagnose seed abnormalities using optical coherence tomography (OCT). Melon seeds infected with Cucumber green mottle mosaic virus (CGMMV) were scanned by OCT. The cross-sectional sensed area of the abnormal seeds showed an additional subsurface layer under the surface which is not found in normal seeds. The presence of CGMMV in the sample was examined by a blind test (n = 140) and compared by the reverse transcription-polymerase chain reaction. The abnormal layers (n = 40) were quantitatively investigated using A-scan sensing analysis and statistical method. By utilizing 3D OCT image reconstruction, we confirmed the distinctive layers on the whole seeds. These results show that OCT with the proposed data processing method can systemically pick up morphological modification induced by viral infection in seeds, and, furthermore, OCT can play an important role in automa