Oral Vaccination of Baculovirus-Expressed VP28 Displays Enhanced Protection against White Spot Syndrome Virus in Penaeus monodon 英文参考文献.docVIP

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Oral Vaccination of Baculovirus-Expressed VP28 Displays Enhanced Protection against White Spot Syndrome Virus in Penaeus monodon 英文参考文献.doc

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Oral Vaccination of Baculovirus-Expressed VP28 Displays Enhanced Protection against White Spot Syndrome Virus in Penaeus monodon 英文参考文献

OralVaccinationofBaculovirus-ExpressedVP28Displays EnhancedProtectionagainstWhiteSpotSyndromeVirus inPenaeusmonodon SyedMusthaqS1,JimmyKwang1,2* 1Animal Health Biotechnology, Temasek Lifesciences Laboratory, National University of Singapore, Singapore, Singapore, 2Department of Microbiology, Faculty of Medicine,NationalUniversityofSingapore,Singapore,Singapore Abstract White Spot Syndrome Virus (WSSV) is an infectious pathogen of shrimp and other crustaceans, and neither effective vaccinesnoradequatetreatmentsarecurrentlyavailable.WSSVisanenvelopeddsDNAvirus,andoneofitsmajorenvelope proteins,VP28,playsapivotalroleinWSSVinfection.InanattempttodevelopavaccineagainstWSSV,weinsertedthe VP28geneintoabaculovirusvectortailoredtoexpressVP28onthebaculovirussurfaceundertheWSSVie1promoter(Bac- VP28). The Bac-VP28 incorporated abundant quantity (65.3 mg/ml) of VP28. Shrimp were treated by oral and immersion vaccinationwitheitherBac-VP28orwild-typebaculovirus(Bac-wt).ThetreatmentwasfollowedbychallengewithWSSV after 3 and 15 days. Bac-VP28 vaccinated shrimp showed significantly higher survival rates (oral: 81.7% and 76.7%; immersion: 75% and68.4%) than Bac-wt or non-treated shrimp (100% mortality). To verify theprotective effects of Bac- VP28,weexaminedinvivoexpressionofVP28byimmunohistochemistryandquantifiedtheWSSVcopynumberbyqPCR.In additiontothat,wequantifiedtheexpressionlevelsshrimpgenesLGBPandSTATbyreal-timeRT-PCRfromthesamples obtained from Bac-VP28 vaccinated shrimp at different duration of vaccine regime. Our findings indicate that oral vaccinationofshrimpwithBac-VP28isanattractivepreventativemeasureagainstWSSVinfectionthatcanbeusedinthe field. Citation:SSM,KwangJ(2011)OralVaccinationofBaculovirus-ExpressedVP28DisplaysEnhancedProtectionagainstWhiteSpotSyndromeVirus in Penaeus monodon.PLoSONE6(11):e26428.doi:10.1371/journal.pone.0026428 Editor:PierreBoudinot,INRA,France ReceivedJuly29,2011;AcceptedSeptember26,2011;PublishedNovember1,2011 Copyright:?2011S,Kwang.T