Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes 英文参考文献.docVIP

Available online /content/9/5/R93 Research article Open Access Vol 9 No 5 Passage and reversal effects on gene expression of bovine meniscal fibrochondrocytes Najmuddin J Gunja and Kyriacos A Athanasiou Department of Bioengineering, Rice University, PO Box 1892, Houston, TX 77251, USA Corresponding author: Kyriacos A Athanasiou, athanasiou@ Received: 9 Mar 2007 Revisions requested: 25 Apr 2007 Revisions received: 5 Sep 2007 Accepted: 13 Sep 2007 Published: 13 Sep 2007 Arthritis Research Therapy 2007, 9:R93 (doi:10.1186/ar2293) This article is online at: /content/9/5/R93 ? 2007 Gunja and Athanasiou; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract The knee meniscus contains a mixed population of cells that exhibit fibroblastic as well as chondrocytic characteristics. Tissue engineering studies and future therapies for the meniscus require a large population of cells that are seeded on scaffolds. To achieve this, monolayer expansion is often used as a technique to increase cell number. However, the phenotype of these cells may be significantly different from that of the primary population. The objective of this study was to investigate changes in meniscal fibrochondrocytes at the gene expression level over four passages using quantitative real-time reverse transcriptase polymerase chain reaction. Cells from the inner addition, primary and passaged meniscus fibrochondrocytes were placed on surfaces coated with collagen I or aggrecan protein to investigate whether any gene expression changes resulting from passage could be reversed. Collagen I expression was found to increase with the number of passages, whereas collagen II and COMP expression decreased. Collage