油菜菌核病菌不同致病力菌株生物学特性的比较研究.PDFVIP

安徽农业大学学报, 2011, 38(6): 916-919 Journal of Anhui Agricultural University [DOI]CNKI：34-1162/S1027.014 网络出版时间：2011-10-25 10:27:22 [URL]/kcms/detail/34.1162.S1027.014.html 油菜菌核病菌不同致病力菌株生物学特性的比较研究 * 凌 萍，高宁馨，高智谋 ，王淋玉，喻海峰 (安徽农业大学植物保护学院，合肥 230036) 摘 要：以从安徽省桐城、望江、宁国、旌德、南陵、寿县等油菜产区采集的油菜菌核病病组织或菌核上分离 获得的 24 株油菜菌核病菌菌株为供试菌株，采用离体叶片接种法接种测定这些菌株对供试油菜的致病力。结果表 明，各菌株间的致病力有显著差异，存在明显分化，这种致病性差异与菌株的地理来源有一定相关。进一步选取致 病力较强的菌株 NG4 和致病力较弱的菌株 NL1 进行生物学特性的比较研究。结果表明，两菌株在 5 ～30 ℃条件 下均能生长，最适生长温度为 20 ～25 ℃；菌丝在pH 2 ～12 均能生长，以 pH 5 ～7 为最适；光暗交替条件有利于 菌丝的生长；但强致病力菌株在相同温度、pH 和光照条件下的菌丝生长速率和菌丝干重均大于弱致病力菌株。 关键词：油菜菌核病菌；致病力；生物学特性；地理来源 中图分类号：S435.654 文献标识码：A 文章编号：1672−352X (2011)06−0916−04 Comparison of the biological characteristics of different pathogenic strains in Sclerotinia sclerotiorum LING Ping, GAO Ning-xin, GAO Zhi-mou, WANG Lin-yu, YU Hai-feng (School of Plant Protection, Anhui Agricultural University, Hefei 230036) Abstract: The pathogenicity of 24 isolates of Sclerotinia sclerotiorum which obtained from Tongcheng, Wangjiang, Ningguo, Jingde, Nanling and Shouxian in Anhui was investigated by applying detached leaf inocula- tion. The results showed that all of the tested isolates caused stem rot on rape, and there was a significant differ- ence in the average length of the lesions caused by different isolates, suggesting that there was significant differ- entiation in pathogenicity of S. sclerotiorum strains to rapeseed. The pathogenicity of the isolates was related to their localities to some degree. Based on the results above, the strong pathonic i