Polyphosphate Kinase 2 A Novel Determinant of Stress Responses and Pathogenesis in Campylobacter jejuni 英文参考文献.docVIP
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Polyphosphate Kinase 2 A Novel Determinant of Stress Responses and Pathogenesis in Campylobacter jejuni 英文参考文献
PolyphosphateKinase2:ANovelDeterminantofStress
ResponsesandPathogenesisinCampylobacterjejuni
DharaneshGangaiah1,ZheLiu1,Jesu′sArcos2,IssmatI.Kassem1,YasserSanad1,JordiB.Torrelles2,
GireeshRajashekara1*
1Food Animal Health Research Program, Ohio Agricultural Research Development Center, Department of Veterinary Preventive Medicine, The Ohio State University,
Wooster,Ohio,UnitedStatesofAmerica,2CenterforMicrobialInterfaceBiology,TheOhioStateUniversity,Columbus,Ohio,UnitedStatesofAmerica
Abstract
Background:Inorganicpolyphosphate(polyP)playsanimportantroleinstresstoleranceandvirulenceinmanybacteria.
PPK1istheprincipalenzymeinvolvedinpolyPsynthesis,whilePPK2usespolyPtogenerateGTP,asignalingmoleculethat
serves as an alternative energy source and a precursor for various physiological processes. Campylobacter jejuni , an
important cause of foodborne gastroenteritis in humans, possesses homologs of both ppk1 and ppk2. ppk1 has been
previouslyshowntoimpactthepathobiologyofC.jejuni.
Methodology/PrincipalFindings:Here,wedemonstrateforthefirsttimethatthedeletionofppk2inC.jejuniresultedina
significantdecreaseinpolyP-dependentGTPsynthesis,whiledisplayinganincreasedintracellularATP:GTPratio.TheDppk2
mutantexhibitedasignificantsurvivaldefectunderosmotic,nutrient,aerobic,andantimicrobialstressesanddisplayedan
enhanced ability to form static biofilms. However, the Dppk2 mutant was not defective in poly P and ppGpp synthesis
suggestingthatPPK2-mediatedstresstoleranceisnotppGpp-mediated.Importantly,theDppk2mutantwassignificantly
attenuatedininvasionandintracellularsurvivalwithinhumanintestinalepithelialcellsaswellasinchickencolonization.
Conclusions/Significance:Takentogether,wehavehighlightedtheroleofPPK2asanovelpathogenicitydeterminantthat
iscriticalforC.jejunisurvival,adaptation,andpersistenceinthehostenvironments.PPK2isabsentinhumansandanimals;
therefore,canserveasanoveltargetfortherapeuticinterventionofC.jejuniinfections.
Citation:GangaiahD,LiuZ,ArcosJ,KassemII,SanadY,etal.(2010
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