Porin new light onto chromatin and nuclear organization 英文参考文献.docVIP

Minireview Porin new light onto chromatin and nuclear organization Lee E Finlan and Wendy A Bickmore Address: MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Crewe Road, Edinburgh, EH4 2XU, UK. Correspondence: Lee E Finlan. Email: lfinlan@hgu.mrc.ac.uk; Wendy A Bickmore Email. w.bickmore@hgu.mrc.ac.uk Published: 16 May 2008 Genome Biology 2008, 9:222 (doi:10.1186/gb-2008-9-5-222) The electronic version of this article is the complete one and can be found online at /2008/9/5/222 ? 2008 BioMed Central Ltd Abstract A recent report identifies sites in the human genome that can associate with nucleoporin 93, a subunit of the nuclear pore complex. These associations are modulated by levels of global histone acetylation and highlight the dynamic nature of chromatin organization in the nucleus. Nuclear pore complexes (NPCs) in the nuclear envelope mediate the selective exchange of macromolecules into and out of the nucleus by facilitated diffusion. The NPC is com- posed of multiple copies of roughly 30 distinct nucleoporins [1-3] and its structure is conserved throughout eukaryotes. Although numerous studies have examined how nucleoporins function in nucleo-cytoplasmic transport, relatively little attention has been paid to any role they might have in influencing genome organization in mammalian cells. nucleoplasmic protein [12]. The Silver lab had previously shown by ChIP that various components of the yeast NPC, including Nic96, the yeast homolog of NUP93, could asso- ciate with specific regions of the yeast genome [13]. They have now extended this approach to regions of three different human chromosomes that interact with NUP93 in HeLa cells [10]. After cell fixation with dimethyl adipimidate and paraformaldehyde, lysis in detergent and then sonica- tion, DNA sequences associating with NUP93 in the soluble extr